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AB279346

Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric)

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Human Recombinant Monoclonal CD30 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

View Alternative Names

CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30

3 Images
Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) (AB279346)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) (AB279346)

Flow cytometric analysis of KARPAS-299 (Human anaplastic large cell lymphoma) cells labelling CD30 with ab279346 at 1/1000 dilution (0.1μg) (Red) compared with a Human IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti human IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) (AB279346)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) (AB279346)

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte) (Left) / HDLM-2 (Human Hodgkin lymphoma) (Right) cells labelling CD30 with ab279346 at 1/1000 dilution (0.1μg) (Red) compared with a Human IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Human IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Negative control : Jurkat.

Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) (AB279346)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) (AB279346)

Immunocytochemical analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 cells labelling CD30 with ab279346 at 1/50 (22.74 μg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in KARPAS-299 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Negative control : Jurkat (PMID : 15920551).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

SH313-B5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>22.74 μg/ml</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<p>0.895 μg/ml</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>22.74 μg/ml</p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<p>0.895 μg/ml</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab279346 is a human monoclonal chimeric antibody.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF8/CD30L (PubMed : 8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed : 8999898).
See full target information TNFRSF8
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com