JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279349

Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Human Recombinant Monoclonal CD30 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

View Alternative Names

CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) - BSA and Azide free (AB279349)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) - BSA and Azide free (AB279349)

This data was developed using ab279346 the same antibody clone in a different buffer formulation.

Immunocytochemical analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 cells labelling CD30 with ab279346 at 1/50 (22.74 μg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in KARPAS-299 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Negative control : Jurkat (PMID : 15920551).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) - BSA and Azide free (AB279349)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) - BSA and Azide free (AB279349)

This data was developed using ab279346 the same antibody clone in a different buffer formulation.

Flow cytometric analysis of KARPAS-299 (Human anaplastic large cell lymphoma) cells labelling CD30 with ab279346 at 1/1000 dilution (0.1μg) (Red) compared with a Human IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti human IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) - BSA and Azide free (AB279349)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric) - BSA and Azide free (AB279349)

This data was developed using ab279346 the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte) (Left) / HDLM-2 (Human Hodgkin lymphoma) (Right) cells labelling CD30 with ab279346 at 1/1000 dilution (0.1μg) (Red) compared with a Human IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Human IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Negative control : Jurkat.

  • Unconjugated

    Anti-CD30 antibody [SH313-B5] - Human IgG1 (Chimeric)

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

SH313-B5

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

This human monoclonal chimeric antibody (ab279349) is the carrier-free version of ab279346.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for TNFSF8/CD30L (PubMed : 8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed : 8999898).
See full target information TNFRSF8
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB236711

Human CD30 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CD30 ELISA Kit (AB236711)

  • 0
  • 0
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 61
Primary Antibodies

AB245756

Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free (AB245756)

  • 0
  • 0
Primary Antibodies

AB52587

Anti-PD1 antibody [NAT105]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (AB52587)

  • 5
  • 9
Primary Antibodies

AB259660

Anti-CD30-L antibody [EPR21920-290] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CD30-L antibody [EPR21920-290] - BSA and Azide free (Detector) (AB259660)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com