Skip to main content

Mouse Recombinant Monoclonal CD30 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Mouse IgG2a (AB279347), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [SH313-B5] - Mouse IgG2a (AB279347), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWB
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898).

Alternative names

Recommended products

Mouse Recombinant Monoclonal CD30 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SH313-B5

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab279347 is a mouse monoclonal chimeric antibody.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Mouse IgG2a (ab279347), expandable thumbnail

    Flow Cytometry - Anti-CD30 antibody [SH313-B5] - Mouse IgG2a (ab279347)

    Flow cytometric analysis of KARPAS-299 (human anaplastic large cell lymphoma) cells labelling CD30 with ab279347 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [SH313-B5] - Mouse IgG2a (ab279347), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [SH313-B5] - Mouse IgG2a (ab279347)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labelling CD30 with ab279347 at 1/50 dilution (17.9 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in KARPAS-299 cell line. Negative control: Jurkat (PMID: 15920551).

    Tubulin counterstain was Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com