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AB53317

Anti-CD300 antibody [MEM-260]

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(1 Publication)

Mouse Monoclonal CD300a antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD300LF protein.

View Alternative Names

CD300a, CMRF35H, IGSF12, HSPC083, CD300A, CMRF35-like molecule 8, CLM-8, CD300 antigen-like family member A, CMRF-35-H9, CMRF35-H, IRC1/IRC2, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CMRF35-H9, IgSF12, IRp60

2 Images
Flow Cytometry - Anti-CD300 antibody [MEM-260] (AB53317)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD300 antibody [MEM-260] (AB53317)

Separation of human monocytes (red-filled) from CD300a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab53317 at 4 µg/ml (GAM APC).

Flow Cytometry - Anti-CD300 antibody [MEM-260] (AB53317)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD300 antibody [MEM-260] (AB53317)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab53317 at 4 µg/ml (GAM APC).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-260

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Cell preparation containing CD300LF protein. The exact immunogen used to generate this antibody is proprietary information.

Q8TDQ1

Specificity

ab53317 recognizes CD300a, (Customer feedback suggests it also cross-reacts with CD300c, however we have not yet tested it for CD300c). The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed : 10746781, PubMed : 16339535, PubMed : 9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed : 22043923).
See full target information CD300A

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical, cosmetic and investigational dermatology 17:1387-1404 PubMed38881700

2024

MALAT1 Knockdown Inhibits the Proliferation, Migration, and Collagen Deposition of Human Hypertrophic Scar Fibroblasts via Targeting miR-29a-3p/Smurf2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Guo,Xiaoxiao Liu,Keqing Qiu,Longxiang Tu,Dewu Liu
View all publications

Product promise

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