Rabbit Recombinant Monoclonal CD300a antibody. Suitable for Dot, Flow Cyt and reacts with Transfected cell line - Human, Human samples.
Images
![Dot Blot - Anti-CD300a antibody [EPR27995-84] (AB320719), expandable thumbnail](https://content.abcam.com/products/images/cd300a-antibody-epr27995-84-ab320719--dot-blot-img435507.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-CD300a antibody [EPR27995-84] (AB320719)")
![Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (AB320719), expandable thumbnail](https://content.abcam.com/products/images/cd300a-antibody-epr27995-84-ab320719--flow-cytometry-img435490.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (AB320719)")
![Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (AB320719), expandable thumbnail](https://content.abcam.com/products/images/cd300a-antibody-epr27995-84-ab320719--flow-cytometry-img435489.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (AB320719)")
![Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (AB320719), expandable thumbnail](https://content.abcam.com/products/images/cd300a-antibody-epr27995-84-ab320719--flow-cytometry-img435488.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (AB320719)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| Dot | Flow Cyt | ICC/IF | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Target data
Function
Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed:10746781, PubMed:16339535, PubMed:9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed:22043923).
Alternative names
CD300a, CMRF35H, IGSF12, HSPC083, CD300A, CMRF35-like molecule 8, CLM-8, CD300 antigen-like family member A, CMRF-35-H9, CMRF35-H, IRC1/IRC2, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CMRF35-H9, IgSF12, IRp60
Recommended products
Rabbit Recombinant Monoclonal CD300a antibody. Suitable for Dot, Flow Cyt and reacts with Transfected cell line - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27995-84
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Dot Blot - Anti-CD300a antibody [EPR27995-84] (ab320719), expandable thumbnail](https://content.abcam.com/products/images/cd300a-antibody-epr27995-84-ab320719--dot-blot-img435507.jpg "Dot Blot - Anti-CD300a antibody [EPR27995-84] (ab320719)")
Dot Blot - Anti-CD300a antibody [EPR27995-84] (ab320719)
Dot blot analysis of CD300a using ab320719 at 1:1000 (0.496 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: 293T cells transfected with a human CD300a expression vector containing a myc-His-tag® whole cell lysate.
Lane 2: 293T cells transfected with a human CD300c expression vector containing a myc-His-tag® whole cell lysate.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with overexpressed human CD300c by dot blot.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Dot Blot - Anti-CD300a antibody [EPR27995-84] (ab320719) at 1/1000 dilution
Lane 1: 293T cells transfected with a human CD300a expression vector containing a myc-His-tag® whole cell lysate
Lane 2: 293T cells transfected with a human CD300c expression vector containing a myc-His-tag® whole cell lysate
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Exposure time: 180s
![Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719), expandable thumbnail](https://content.abcam.com/products/images/cd300a-antibody-epr27995-84-ab320719--flow-cytometry-img435490.jpg "Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719)")
Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719)
Flow cytometric analysis of THP-1 (human monocytic leukemia monocyte) (Right) / 293T (human embryonic kidney epithelial cell) (Left) cells labelling CD300a with ab320719 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: 293T (PMID: 22043923).![Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719), expandable thumbnail](https://content.abcam.com/products/images/cd300a-antibody-epr27995-84-ab320719--flow-cytometry-img435489.jpg "Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719)")
Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab320719 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti human CD19 conjugated to PE/Cy7.
Gated on viable Lymphocytes.![Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719), expandable thumbnail](https://content.abcam.com/products/images/cd300a-antibody-epr27995-84-ab320719--flow-cytometry-img435488.jpg "Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719)")
Flow Cytometry - Anti-CD300a antibody [EPR27995-84] (ab320719)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab320719 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti human CD14 conjugated to Brilliant Violet 421.
Gated on viable cells.
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