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Rabbit Recombinant Monoclonal CD300e antibody. Suitable for Dot, Flow Cyt, ICC/IF and reacts with Recombinant fragment - Human, Human samples.

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Images

Dot Blot - Anti-CD300e antibody [EPR30141-593] (AB322924), expandable thumbnail
  • Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (AB322924), expandable thumbnail
  • Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (AB322924), expandable thumbnail
  • Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (AB322924), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD300e antibody [EPR30141-593] (AB322924), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotFlow CytICC/IFIHC-PWB
Human
Expected
Tested
Tested
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Probably acts as an activating receptor.

Alternative names

Rabbit Recombinant Monoclonal CD300e antibody. Suitable for Dot, Flow Cyt, ICC/IF and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR30141-593

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Dot Blot - Anti-CD300e antibody [EPR30141-593] (ab322924), expandable thumbnail

    Dot Blot - Anti-CD300e antibody [EPR30141-593] (ab322924)

    Dot blot analysis of CD300e using ab322924 at 1000 (0.523 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: No-tagged human CD300e recombinant fragment

    Lane2: His-tagged human CD300a recombinant fragment

    Lane3: His-tagged human CD300c recombinant fragment

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human CD300a and CD300c.

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-CD300e antibody [EPR30141-593] (ab322924) at 1/1000 dilution

    Lane 1: No-tagged human CD300e recombinant fragment

    Lane 2: His-tagged human CD300a recombinant fragment

    Lane 3: His-tagged human CD300c recombinant fragment

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (ab322924), expandable thumbnail

    Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (ab322924)

    Flow cytometric analysis of Human PBMC(human peripheral blood mononuclear cell) cells labelling CD300e with ab322924 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Human PBMC were co-stained with CD19 conjugated PE/Cy7 .

    Gated on viable cell.

  • Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (ab322924), expandable thumbnail

    Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (ab322924)

    Flow cytometric analysis of Human PBMC(human peripheral blood mononuclear cell) cells labelling CD300e with ab322924 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Human PBMC were co-stained with CD3 conjugated Alexa Fluor®647.

    Gated on viable cell.

  • Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (ab322924), expandable thumbnail

    Flow Cytometry - Anti-CD300e antibody [EPR30141-593] (ab322924)

    Flow cytometric analysis of Human PBMC(human peripheral blood mononuclear cell) cells labelling CD300e with ab322924 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Human PBMC were co-stained with CD14 conjugated BV421.

    Gated on viable cell.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD300e antibody [EPR30141-593] (ab322924), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD300e antibody [EPR30141-593] (ab322924)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (Human primary peripheral blood mononuclear cell) cells labelling CD300e with ab322924 at 1/50 (10.46 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in subsets of CD3 negative human PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-human CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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