Rat Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples. Cited in 1 publication.
Images
![Flow Cytometry - Anti-CD31 antibody [13.3] - BSA and Azide free (AB256573), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-133-bsa-and-azide-free-ab256573--flow-cytometry-img70541.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD31 antibody [13.3] - BSA and Azide free (AB256573)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [13.3] - BSA and Azide free (AB256573), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-133-bsa-and-azide-free-ab256573--immunocytochemistry-immunofluorescence-img22538.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [13.3] - BSA and Azide free (AB256573)")
Publications
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- European journal of cell biology 63:247-541994Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Vecchi,C Garlanda,M G Lampugnani,M Resnati,C Matteucci,A Stoppacciaro,H Schnurch,W Risau,L Ruco,A MantovaniPubMed 8082649
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).
Alternative names
CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1
Recommended products
Rat Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 13.3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256573 is the carrier-free version of Anti-CD31 antibody [MEC 13.3] ab256569.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CD31 antibody [13.3] - BSA and Azide free (ab256573), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-133-bsa-and-azide-free-ab256573--flow-cytometry-img70541.jpg "Flow Cytometry - Anti-CD31 antibody [13.3] - BSA and Azide free (ab256573)")
Flow Cytometry - Anti-CD31 antibody [13.3] - BSA and Azide free (ab256573)
Flow cytometric analysis of B16-F0 (mouse melanoma epithelial cell-like) (Left panel) / bEnd.3 (mouse brain endothelioma) (Right panel) cells labelling CD31 with Anti-CD31 antibody [MEC 13.3] ab256569 at 1/1000 dilution (1.058μg/ml) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
Negative control: B16-F0 (PMID: 8082649).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [MEC 13.3] ab256569).
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [13.3] - BSA and Azide free (ab256573), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-133-bsa-and-azide-free-ab256573--immunocytochemistry-immunofluorescence-img22538.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [13.3] - BSA and Azide free (ab256573)")
Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [13.3] - BSA and Azide free (ab256573)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized b.end3 and B16-F0(-) cells labelling CD31 with Anti-CD31 antibody [MEC 13.3] ab256569 at 1/50 dilution (21.16μg/ml), followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2μg/ml) (Green). Confocal image showing membranous and cytoplasmic staining in b.end3 cells.
Negative control: B16-F0 (PMID: 8082649).
Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4μg/ml) (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: Anti-CD31 antibody [MEC 13.3] ab256569 at a 1/50 dilution (21.16μg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/500 dilution (4μg/ml).
Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 at 1/200 dilution (2.5μg/ml) followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 at a 1/1000 dilution (2μg/ml).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [MEC 13.3] ab256569).
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