Anti-CD31 antibody [13.3] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [13.3] - BSA and Azide free (AB256573)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized b.end3 and B16-F0(-) cells labelling CD31 with ab256569 at 1/50 dilution (21.16μg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (2μg/ml) (Green). Confocal image showing membranous and cytoplasmic staining in b.end3 cells.

Negative control : B16-F0 (PMID : 8082649).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5μg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (4μg/ml) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab256569 at a 1/50 dilution (21.16μg/ml) followed by ab150080 at a 1/500 dilution (4μg/ml).

Negative control 2 : ab179513 at 1/200 dilution (2.5μg/ml) followed by ab150157 at a 1/1000 dilution (2μg/ml).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256569).

• Flow Cyt

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Flow Cytometry - Anti-CD31 antibody [13.3] - BSA and Azide free (AB256573)

Flow cytometric analysis of B16-F0 (mouse melanoma epithelial cell-like) (Left panel) / bEnd.3 (mouse brain endothelioma) (Right panel) cells labelling CD31 with ab256569 at 1/1000 dilution (1.058μg/ml) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor^® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

Negative control : B16-F0 (PMID : 8082649).

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256569).