Anti-CD31 antibody ab28364 is a rabbit polyclonal antibody that is used in CD31 IHC. Suitable for human samples.
- Tried and trusted by researchers since 2006
Same trusted quality, new lower price
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- Renal failure 46:23007272024CXC chemokine receptor 7 ameliorates renal fibrosis by inhibiting β-catenin signaling and epithelial-to-mesenchymal transition in tubular epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Meng,Chunli Liu,Jingchun Li,Ping Fang,Bo Yang,Wei Sun,Yunfang ZhangPubMed 38189094
- Nature communications 14:81722023The long non-coding RNA HOTAIR contributes to joint-specific gene expression in rheumatoid arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuriel Elhai,Raphael Micheroli,Miranda Houtman,Masoumeh Mirrahimi,Larissa Moser,Chantal Pauli,Kristina Bürki,Andrea Laimbacher,Gabriela Kania,Kerstin Klein,Philipp Schätzle,Mojca Frank Bertoncelj,Sam G Edalat,Leandra Keusch,Alexandra Khmelevskaya,Melpomeni Toitou,Celina Geiss,Thomas Rauer,Maria Sakkou,George Kollias,Marietta Armaka,Oliver Distler,Caroline OspeltPubMed 38071204
- Nature communications 14:80972023SEMA6A drives GnRH neuron-dependent puberty onset by tuning median eminence vascular permeability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonella Lettieri,Roberto Oleari,Marleen Hester van den Munkhof,Eljo Yvette van Battum,Marieke Geerte Verhagen,Carlotta Tacconi,Marco Spreafico,Alyssa Julia Jennifer Paganoni,Roberta Azzarelli,Valentina Andre',Federica Amoruso,Luca Palazzolo,Ivano Eberini,Leo Dunkel,Sasha Rose Howard,Alessandro Fantin,Ronald Jeroen Pasterkamp,Anna CariboniPubMed 38062045
- Acta biochimica Polonica 70:843-8532023Modified Hongyu Decoction promotes wound healing by activating the VEGF/PI3K/Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Xu,Wei-Hua Yang,Zhi-Wei Miao,Chun-Yu Zhang,Yi-Jia Cheng,Yang Chen,Jin-Gen Lu,Ning HePubMed 38051819
- International journal of nanomedicine 18:7379-74022023Nanocurcumin Reduces High Glucose and Particulate Matter-Induced Endothelial Inflammation: Mitochondrial Function and Involvement of miR-221/222.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsai-Chun Lai,Chiang-Wen Lee,Mei-Hsiang Hsu,Yu-Chen Chen,Shu-Rung Lin,Shu-Wha Lin,Tzu-Lin Lee,Shin-Yu Lin,Shu-Hao Hsu,Jaw-Shiun Tsai,Yuh-Lien ChenPubMed 38084125
- Bioactive materials 33:114-1282023Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoungnam Kang,Miji Yeo,Irem Deniz Derman,Dino J Ravnic,Yogendra Pratap Singh,Mecit Altan Alioglu,Yang Wu,Jasson Makkar,Ryan R Driskell,Ibrahim T OzbolatPubMed 38024230
- Nature communications 14:78632023SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKi-Fong Man,Lei Zhou,Huajian Yu,Ka-Hei Lam,Wei Cheng,Jun Yu,Terence K Lee,Jing-Ping Yun,Xin-Yuan Guan,Ming Liu,Stephanie MaPubMed 38030644
- Journal of ovarian research 16:2312023BRCA1 and BRCA2 deficient tumour models generate distinct ovarian tumour microenvironments and differential responses to therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSalar Farokhi Boroujeni,Galaxia Rodriguez,Kristianne Galpin,Edward Yakubovich,Humaira Murshed,Dalia Ibrahim,Sara Asif,Barbara C VanderhydenPubMed 38017453
- Nature communications 14:77832023Local H release remodels senescence microenvironment for improved repair of injured bone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengqiang Chen,Yuanman Yu,Songqing Xie,Danna Liang,Wei Shi,Sizhen Chen,Guanglin Li,Wei Tang,Changsheng Liu,Qianjun HePubMed 38012166
- Pediatric surgery international 40:72023Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianna Scuglia,Laura P Frazão,Alice Miranda,Albino Martins,Joana Barbosa-Sequeira,Diana Coimbra,Adhemar Longatto-Filho,Rui L Reis,Cristina Nogueira-Silva,Nuno M Neves,Jorge Correia-PintoPubMed 37999778
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig | Dilution info - | Notes - |
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Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity).Isoform Delta15Does not protect against apoptosis.
Alternative names
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
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Anti-CD31 antibody ab28364 is a rabbit polyclonal antibody that is used in CD31 IHC. Suitable for human samples.
- Tried and trusted by researchers since 2006
Same trusted quality, new lower price
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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6 product images
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)
Immunohistochemistry (PFA-fixed paraffin-embedded sections) analysis of human palatine tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (15 hours at 4°C). Heat mediated antigen retrieval was performed using Tris/EDTA buffer. Blocking performed with 5% normal goat serum for 1 hour at 21°C. HRP-conjugated Goat Anti-Rabbit was used as the secondary antibody.
This image is courtesy of a customer review submitted by Mr. Victor Bernal.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (30 minutes at 23°C). 10% Neutral buffered formalin used as fixative. Heat mediated antigen retrieval was performed at 95°C for 20 minutes. Blocking performed using 2.5% serum for 20 minutes at 23°C. HRP-conjugated Goat Anti-rabbit was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)
ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)
Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling CD31 with ab28364 at a concentration of 0.40 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab28364 Anti-CD31 antibody was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)
Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling CD31 with ab28364 at a concentration of 0.50 µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.ab28364 Anti-CD31 antibody was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human skin tissue. Stained with ab28364 at 1/100 dilution. Secondary antibody used was Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG at 1/300 dilution. Blocking was done with 5% BSA for 30 minutes at 21°C. The sample was incubated with the primary antibody for 30 minutes at 4°C. Antigen retrieval method was heat mediated with citrate buffer pH 6
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