AB218477

Anti-CD31 antibody [C31/1395R]

Recombinant
Lab Essentials
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CD31 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PECAM1.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31/1395R] (AB218477)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31/1395R] (AB218477)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human angiosarcoma tissue labeling CD31 with ab218477 at 2μg/ml.

Supplier Data

Western blot - Anti-CD31 antibody [C31/1395R] (AB218477)

All lanes:

Western blot - Anti-CD31 antibody [C31/1395R] (ab218477) at 2 µg/mL

All lanes:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Predicted band size: 82 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

C31/1395R

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PECAM1.

P16284

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.Positive Control: Hu tonsil tissue" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab218477 is purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.

See full target information PECAM1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The European journal of neuroscience 62:e70235 PubMed40960371

2025

Electrical Stimulation-Induced Muscle Damage Alters Hippocampal BDNF Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Wirtz,Rémi Chaney,Marina Cefis,Alexandre Méloux,Yuan Wang,Stéphanie Lemaire,Aurore Quirié,Julien Delezie,Gilles Gouspillou,Anne Prigent-Tessier,Philippe Garnier

View all publications

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