AB212709

Anti-CD31 antibody [C31.3] - BSA and Azide free

Name: Anti-CD31 antibody [C31.3] - BSA and Azide free (ab212709) | Abcam
Brand: Abcam Limited
SKU: AB212709
Price: 905 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD31 antibody. Carrier free. Suitable for ICC, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PECAM1.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3] - BSA and Azide free (AB212709)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3] - BSA and Azide free (AB212709)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human angiosarcoma tissue labeling CD31 with ab212709 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3] - BSA and Azide free (AB212709)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD31 with ab212709 at 2 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C31.3

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PECAM1.

P16284

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "0.5-1 µg/mL", "ICC-species-notes": "Use 0.3M glycine to quench autofluorescence caused by aldehydes.Positive Control: HUVEC cells", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Incubate for 30 minutes at RT.The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.Positive Control: Hu tonsil tissue" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab212709 is purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.

See full target information PECAM1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 6: PubMed29025747

2017

Uncoupling Protein 2 Inhibits Myointimal Hyperplasia in Preclinical Animal Models of Vascular Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Yaolei Zhang,Wei Li,Peijian Wang,Rui Gu,Yaxing Feng,Shujie Wei,Ke Peng,Yunrong Zhang,Linan Su,Qiang Wang,De Li,Dachun Yang,Wing Tak Wong,Yongjian Yang,Shuangtao Ma

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