Mouse Multiclonal CD31 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Tissue within Human PECAM1.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-ab199012--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73843.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)")
![Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-ab199012--western-blot-img101793.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-ab199012--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139548.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)")
Publications
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- Journal of nanobiotechnology 21:2942023Functional molecule-mediated assembled copper nanozymes for diabetic wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyan Huang,Ping Xu,Xiaoxue Fu,Jiaxin Yang,Weihong Jing,Yucen Cai,Yingjuan Zhou,Rui Tao,Zhangyou YangPubMed 37626334
- Cells 12:2023Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Alleviates Atherosclerotic Plaque by Inhibiting Ox-LDL Uptake, Inflammatory Reaction and Endothelial Damage in Rabbits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhong Li,Guiying Shi,Wei Liang,Haiquan Shang,Huiwu Li,Yunlin Han,Wenjie Zhao,Lin Bai,Chuan QinPubMed 37566014
- Nature communications 12:57612021Intrathymic differentiation of natural antibody-producing plasma cells in human neonates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHector Cordero,Rodney G King,Pranay Dogra,Chloe Dufeu,Sarah B See,Alexander M Chong,Anne-Catrin Uhlemann,Siu-Hong Ho,David M Kalfa,Emile A Bacha,John F Kearney,Emmanuel ZornPubMed 34599177
- Stem cell research & therapy 12:4072021Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhong Li,Guiying Shi,Yunlin Han,Haiquan Shang,Huiwu Li,Wei Liang,Wenjie Zhao,Lin Bai,Chuan QinPubMed 34266502
- Theranostics 11:6461-64762021Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingyang Li,Zhenlai Zhu,Lei Wang,Yiting Lin,Hui Fang,Jie Lei,Tianyu Cao,Gang Wang,Erle DangPubMed 33995668
- Bioactive materials 6:1827-18382020Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiu-Zhen Zhang,Yan-Lin Jiang,Jun-Gen Hu,Long-Mei Zhao,Qiu-Zhu Chen,Yan Liang,Yi Zhang,Xiong-Xin Lei,Rui Wang,Yi Lei,Qing-Yi Zhang,Jesse Li-Ling,Hui-Qi XiePubMed 33336114
- Translational vision science & technology 9:62020Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Chi Liu et. al.PubMed 32818094
- IEEE transactions on ultrasonics, ferroelectrics, 67:1725-17292020Validation of Ultrasound Super-Resolution Imaging of Vasa Vasorum in Rabbit Atherosclerotic Plaques.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiyang Chen et. al.PubMed 32086204
- ACS applied materials & interfaces 10:42146-421542018Integrating 3D Printing and Biomimetic Mineralization for Personalized Enhanced Osteogenesis, Angiogenesis, and Osteointegration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLimin Ma et. al.PubMed 30507136
- JCI insight 3:2018Imbalance of immunological synapse-kinapse states reflects tumor escape to immunity in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura R Díaz et. al.PubMed 30232280
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- Recombinant Tissue within Human PECAM1. The exact immunogen used to generate this antibody is proprietary information. Database link P16284
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue |
Associated Products
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8 products for Alternative Product
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.
Alternative names
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Mouse Multiclonal CD31 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Tissue within Human PECAM1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- Recombinant Tissue within Human PECAM1. The exact immunogen used to generate this antibody is proprietary information. Database link P16284
- Clone number
- C31.3 + JC/70A
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
ab199012 was purified from Bioreactor Concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-ab199012--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73843.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CD31 with ab199012.
![Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-ab199012--western-blot-img101793.jpg "Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012)")
Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012)
All lanes: Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012) at 2 µg/mL
All lanes: THP-1 (human monocytic leukemia cell line) whole cell lysate
Predicted band size: 82 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-ab199012--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139548.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human angiosarcoma tissue labelling CD31 with ab199012.
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