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AB199012

Anti-CD31 antibody [C31.3 + JC/70A]

4

(4 Reviews)

|

(19 Publications)

Mouse Multiclonal CD31 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Tissue within Human PECAM1.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CD31 with ab199012.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human angiosarcoma tissue labelling CD31 with ab199012.

Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)
  • WB

Supplier Data

Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (AB199012)

All lanes:

Western blot - Anti-CD31 antibody [C31.3 + JC/70A] (ab199012) at 2 µg/mL

All lanes:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Predicted band size: 82 kDa

false

Key facts

Host species

Mouse

Clonality

Multiclonal

Clone number

C31.3 + JC/70A

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Tissue within Human PECAM1. The exact immunogen used to generate this antibody is proprietary information.

P16284

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.</p><p>The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.</p><p>Positive Control: Hu tonsil tissue</p>" } } }
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Product details

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab199012 was purified from Bioreactor Concentrate.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information PECAM1
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 21:294 PubMed37626334

2023

Functional molecule-mediated assembled copper nanozymes for diabetic wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Huang,Ping Xu,Xiaoxue Fu,Jiaxin Yang,Weihong Jing,Yucen Cai,Yingjuan Zhou,Rui Tao,Zhangyou Yang

Cells 12: PubMed37566014

2023

Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Alleviates Atherosclerotic Plaque by Inhibiting Ox-LDL Uptake, Inflammatory Reaction and Endothelial Damage in Rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhong Li,Guiying Shi,Wei Liang,Haiquan Shang,Huiwu Li,Yunlin Han,Wenjie Zhao,Lin Bai,Chuan Qin

Pharmaceutics 15: PubMed37376106

2023

Phosphorylcholine and KR12-Containing Corneal Implants in HSV-1-Infected Rabbit Corneas.

Applications

Unspecified application

Species

Unspecified reactive species

Kamal Malhotra,Oleksiy Buznyk,Mohammad Mirazul Islam,Elle Edin,Sankar Basu,Marc Groleau,Delali Shana Dégué,Per Fagerholm,Adrien Fois,Sylvie Lesage,Jaganmohan R Jangamreddy,Egidijus Šimoliūnas,Aneta Liszka,Hirak K Patra,May Griffith

Nature communications 12:5761 PubMed34599177

2021

Intrathymic differentiation of natural antibody-producing plasma cells in human neonates.

Applications

Unspecified application

Species

Unspecified reactive species

Hector Cordero,Rodney G King,Pranay Dogra,Chloe Dufeu,Sarah B See,Alexander M Chong,Anne-Catrin Uhlemann,Siu-Hong Ho,David M Kalfa,Emile A Bacha,John F Kearney,Emmanuel Zorn

Stem cell research & therapy 12:407 PubMed34266502

2021

Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhong Li,Guiying Shi,Yunlin Han,Haiquan Shang,Huiwu Li,Wei Liang,Wenjie Zhao,Lin Bai,Chuan Qin

Theranostics 11:6461-6476 PubMed33995668

2021

Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyang Li,Zhenlai Zhu,Lei Wang,Yiting Lin,Hui Fang,Jie Lei,Tianyu Cao,Gang Wang,Erle Dang

Bioactive materials 6:1827-1838 PubMed33336114

2020

Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Zhen Zhang,Yan-Lin Jiang,Jun-Gen Hu,Long-Mei Zhao,Qiu-Zhu Chen,Yan Liang,Yi Zhang,Xiong-Xin Lei,Rui Wang,Yi Lei,Qing-Yi Zhang,Jesse Li-Ling,Hui-Qi Xie

Translational vision science & technology 9:6 PubMed32818094

2020

Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chi Liu,Brad Hall,Nyein Chan Lwin,Ericia Pei Wen Teo,Gary Hin Fai Yam,AnnMarie Hipsley,Jodhbir S Mehta

IEEE transactions on ultrasonics, ferroelectrics, 67:1725-1729 PubMed32086204

2020

Validation of Ultrasound Super-Resolution Imaging of Vasa Vasorum in Rabbit Atherosclerotic Plaques.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyang Chen,Jaesok Yu,Lyudmila Lukashova,Joseph D Latoche,Jianhui Zhu,Linda Lavery,Konstantinos Verdelis,Carolyn J Anderson,Kang Kim

ACS applied materials & interfaces 10:42146-42154 PubMed30507136

2018

Integrating 3D Printing and Biomimetic Mineralization for Personalized Enhanced Osteogenesis, Angiogenesis, and Osteointegration.

Applications

Unspecified application

Species

Unspecified reactive species

Limin Ma,Xiaolan Wang,Naru Zhao,Ye Zhu,Zhiye Qiu,Qingtao Li,Ye Zhou,Zefeng Lin,Xiang Li,Xiaolong Zeng,Hong Xia,Shizhen Zhong,Yu Zhang,Yingjun Wang,Chuanbin Mao
View all publications
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