Mouse Multiclonal CD31 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (AB212711), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-bsa-and-azide-free-ab212711--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97778.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (AB212711)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (AB212711), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-bsa-and-azide-free-ab212711--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140505.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (AB212711)")
Key facts
- Isotype
IgG
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Multiclonal
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Cynomolgus monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
8 products for Alternative Product
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity).Isoform Delta15Does not protect against apoptosis.
Alternative names
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Mouse Multiclonal CD31 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Multiclonal
- Carrier free
Yes
- Clone number
C31.3 + JC/70A
- Purification technique
Affinity purification Protein A/G
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (ab212711), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-bsa-and-azide-free-ab212711--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97778.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (ab212711)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (ab212711)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling CD31 with ab212711 at 2 μg/ml.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (ab212711), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-c313-jc-70a-bsa-and-azide-free-ab212711--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140505.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (ab212711)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [C31.3 + JC/70A] - BSA and Azide free (ab212711)
Immunohistochemical analysis of paraffin-embedded Human angiosarcoma tissue labeling CD31 with ab212711 at 2 μg/ml dilution.
Downloads
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