AB134168

Anti-CD31 antibody [EP3095]

Name: Anti-CD31 antibody [EP3095] (ab134168) | Abcam
Brand: Abcam Limited
SKU: AB134168
Price: 245 USD
Availability: InStock
Rating: 4 (7 reviews)

RabMAb
Recombinant
Lab Essentials
What is this?

(7 Reviews)

(59 Publications)

Anti-CD31 antibody [EP3095] (ab134168) is a rabbit monoclonal antibody detecting CD31 in Western Blot, Flow Cytometry (Intra), IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue, staining CD31 using ab134168 at a 1/250 dilution. Note membrane staining of endothelial cells.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma vessels tissue using ab134168 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-CD31 antibody [EP3095] (AB134168)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD31 antibody [EP3095] (AB134168)

Overlay histogram showing Jurkat cells stained with ab134168 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134168, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemical analysis of paraffin embedded normal Human spleen vessels tissue using ab134168 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemical analysis of paraffin embedded normal Human lung tissue using ab134168 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, staining CD31 using ab134168 at a 1/250 dilution. Note membrane staining of endothelial cells.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab134168 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CD31 antibody [EP3095] (AB134168)

All lanes:

Western blot - Anti-CD31 antibody [EP3095] (ab134168) at 1/1000 dilution

Lane 1:

THP1 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 82 kDa

true

Carrier free

Anti-CD31 antibody [EP3095] - BSA and Azide free
Carrier free

Anti-CD31 antibody [EP3095] - Low endotoxin, Azide free
578 PE

PE Anti-CD31 antibody [EP3095]
660 APC

APC Anti-CD31 antibody [EP3095]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD31 antibody [EP3095]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD31 antibody [EP3095]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD31 antibody [EP3095]
HRP

HRP Anti-CD31 antibody [EP3095]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD31 antibody [EP3095]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3095

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody gave positive results in mouse and rat tissue staining via IHC-P, but we have had difficulties obtaining consistent positive results in WB with mouse and rat samples. Thus, at this time we unfortunately cannot guarantee this antibody to recognize mouse and rat CD31, and optimization may be necessary when working with these species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.Hu Isoform 1-6: 79-83 kDa (predicted)Observed band size is around 130 kDaPositive Control: HUVEC and Jurkat cell lysates (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>); Human spleen and kidney tissue lysate.Negative Control: NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>).", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

What is this antibody validated in?
Anti-CD31 antibody [EP3095] (ab134168) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of CD31?
Anti-CD31 [EP3095] (ab134168) specifically detects a band for CD31 (UniProt: P16284) at a molecular weight of 82kDa.

Trusted by the scientific community
Anti-CD31 [EP3095] (ab134168) was first used in a scientific publication in 2012 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-CD31 [EP3095] (ab134168) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP3095] also available for your convenience: ab134168, Carrier free - ab216459, Carrier free - ab226157, PE - ab305782, APC - ab305783, HRP - ab305784, Alkaline Phosphatase - ab308935, Alexa Fluor® 488 - ab309873, Alexa Fluor® 647 - ab310240, Alexa Fluor® 594 - ab310691, Alexa Fluor® 555 - ab312221

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.

See full target information PECAM1

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Publications (59)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 34:102234 PubMed40893347

2025

Cationic microbubble loading hSIRT3 and hTIMP3 optimize cardiac-targeted delivery and myocardial protection in the porcine MI/R model.

Applications

Unspecified application

Species

Unspecified reactive species

Peian Cai,Kegong Chen,Xionghai Qin,Xingpei Jiang,Xuan Jiao,Kexun Liu,Erliang Guo,Zipeng Li,Xianxin Qiu,Chang Liu,Lu Sun,Junbo Chuai,Jie Wu,Wei Chen,Hai Tian

Nature medicine 31:3451-3463 PubMed40841487

2025

Perioperative IDH inhibition in treatment-naive IDH-mutant glioma: a pilot trial.

Applications

Unspecified application

Species

Unspecified reactive species

Katharine J Drummond,Montana Spiteri,Sarah A Cain,Jordan Jones,Sammy Shaya,Monique Topp,Tianyao Lu,Robert Tobler,Adam L Valkovic,Zachery Moore,Oluwaseun E Fatunla,Jurgen Kriel,Joel J D Moffet,Heidi McAlpine,Marius Rosier,Hefei Guan,James Dimou,Verena Schadewaldt,Samuel Roberts-Thomson,David McArdle,Elaine Lui,Moritz Voelker-Albert,Simone di Sanzo,Brunda Nijagal,Vinod K Narayana,Camilla B Mitchell,Joseph H A Vissers,Sean Grimmond,Mark A Rosenthal,Lucy M Palmer,Sarah A Best,Saskia Freytag,James R Whittle

CNS neuroscience & therapeutics 31:e70439 PubMed40376919

2025

Auricular Transcutaneous Vagus Nerve Stimulation Enhances Post-Stroke Neurological and Cognitive Recovery in Mice by Suppressing Ferroptosis Through α7 Nicotinic Acetylcholine Receptor Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Gong,Fang Zheng,Bochao Niu,Bin Wang,Lin Xu,Yunchao Yang,Jiahan Wang,Xiaopeng Tang,Yanlin Bi

NPJ Regenerative medicine 10:20 PubMed40263309

2025

CYR61 delivery promotes angiogenesis during bone fracture repair.

Applications

Unspecified application

Species

Unspecified reactive species

Annemarie Lang,Emily A Eastburn,Mousa Younesi,Madhura P Nijsure,Carly Siciliano,Annapurna Pranatharthi Haran,Christopher J Panebianco,Elizabeth Seidl,Rui Tang,Eben Alsberg,Nick J Willett,Riccardo Gottardi,Dongeun Huh,Joel D Boerckel

Acta pharmaceutica Sinica. B 15:1143-1158 PubMed40177554

2025

Modulating active targeting nanoparticle design according to tumor progressions.

Applications

Unspecified application

Species

Unspecified reactive species

Huifang Nie,Rong Huang,Guangwei Jiang,Wenshuai Li,Lan Yang,Meng Zhang,Min Qian,Wei Guo,Tao Ye,Rongqin Huang

PLoS pathogens 20:e1012768 PubMed39652618

2024

Fatal Lassa fever in cynomolgus monkeys is associated with systemic viral dissemination and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jimmy Hortion,Emeline Perthame,Blaise Lafoux,Laura Soyer,Stéphanie Reynard,Alexandra Journeaux,Clara Germain,Hélène Lopez-Maestre,Natalia Pietrosemoli,Nicolas Baillet,Séverine Croze,Catherine Rey,Catherine Legras-Lachuer,Sylvain Baize

Stem cell research & therapy 15:388 PubMed39482744

2024

Identification of CD141vasculogenic precursor cells from human bone marrow and their endothelial engagement in the arteriogenesis by co-transplantation with mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gabee Park,Dae Yeon Hwang,Do Young Kim,Ji Young Han,Euiseon Lee,Hwakyung Hwang,Jeong Seop Park,Dae Wook Kim,Seonmin Hong,Sung Vin Yim,Hyun Sook Hong,Youngsook Son

International journal of biological macromolecules :127726 PubMed39491139

2024

A uniform-unsaturated crosslinking strategy to construct injectable alginate hydrogel.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Zhang,Yonggan Yan,Zhao Li,Jing Du,Kai Zhang,Liguo Zhang,Ting Wang,Alberto Bianco,Shaohua Ge,Baojin Ma

Acta neuropathologica 147:70 PubMed38598053

2024

Rare genetic variation in fibronectin 1 (FN1) protects against APOEε4 in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Prabesh Bhattarai,Tamil Iniyan Gunasekaran,Michael E Belloy,Dolly Reyes-Dumeyer,Dörthe Jülich,Hüseyin Tayran,Elanur Yilmaz,Delaney Flaherty,Bengisu Turgutalp,Gauthaman Sukumar,Camille Alba,Elisa Martinez McGrath,Daniel N Hupalo,Dagmar Bacikova,Yann Le Guen,Rafael Lantigua,Martin Medrano,Diones Rivera,Patricia Recio,Tal Nuriel,Nilüfer Ertekin-Taner,Andrew F Teich,Dennis W Dickson,Scott Holley,Michael Greicius,Clifton L Dalgard,Michael Zody,Richard Mayeux,Caghan Kizil,Badri N Vardarajan

Chinese medicine 19:38 PubMed38429819

2024

Traditional Chinese Medicine Shi-Bi-Man ameliorates psoriasis via inhibiting IL-23/Th17 axis and CXCL16-mediated endothelial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Zhang,Xinran Cao,Lixin Zhao,Zitong Ni,Haojie Du,Jiao Qu,Jianxia Zhu,Haiyan Sun,Yang Sun,Zijun Ouyang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD31 antibody [EP3095]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Flow Cytometry (Intracellular) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

Western blot - Anti-CD31 antibody [EP3095] (AB134168)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (59)

Product promise