Name: Anti-CD31 antibody [EPR17259]
SKU: ab182981
Rating: 5 (20 reviews)

Anti-CD31 antibody [EPR17259] (ab182981) is a rabbit monoclonal antibody detecting CD31 in IHC-P, mIHC. Suitable for Human, Mouse, Rat.

- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents

- Biophysical QC for unrivalled batch-batch consistency

- Over 220 publications

Images

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] (AB182981), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product promiseTestedExpectedPredictedNot recommended

	mIHC	IHC-P
Human	Tested	Tested
Mouse	Tested	Tested
Rat	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/4000	Notes -
Species Mouse	Dilution info 1/5000	Notes -
Species Rat	Dilution info 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes In our hands we observed non-specific cytoplasmic staining on tubular cells in rat kidney. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes In our hands we observed non-specific cytoplasmic staining on tubular cells in rat kidney. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes In our hands we observed non-specific cytoplasmic staining on tubular cells in rat kidney. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

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Target data

See full target information PECAM1

Function

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.

Alternative names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17259
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-CD31 antibody [EPR17259] (ab182981) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P and mIHC.

Anti-CD31 antibody [EPR17259] (ab182981) was first used in a scientific publication in 2018 and has been cited over 224 times in peer reviewed journals. It's performance in IHC in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-CD31 antibody [EPR17259] (ab182981) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-CD31 antibody [EPR17259] (ab182981) has 19 independent reviews from customers.

Anti-CD31 antibody [EPR17259] (ab182981) specifically detects CD31 (UniProt ID: Q08481; Molecular weight: 80kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Conjugation-ready, carrier free format available for antibody clone EPR17259 - Anti-CD31 antibody [EPR17259] - BSA and Azide free ab225883.

Antibody clone EPR17259 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor^® 647, Alexa Fluor^® 488, Alexa Fluor^® 555 (Alexa Fluor® 647 Anti-CD31 antibody [EPR17259] ab305210, Alexa Fluor® 488 Anti-CD31 antibody [EPR17259] ab305267, Alexa Fluor® 555 Anti-CD31 antibody [EPR17259] ab312892).

CD31 (PECAM-1) is a protein expressed on endothelial and immune cells, playing a crucial role in tumor angiogenesis and immune cell trafficking. In immuno-oncology, it influences the tumor microenvironment and modulates the immune response to cancer.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD31 also known as Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) is a protein with a mass of approximately 130 kDa. This protein is an important component of the cell surface and is expressed mainly in endothelial cells and certain leukocyte populations. It plays a role in cell-cell adhesion and is recognized as an important marker in immunohistochemistry (IHC) for identifying endothelial cells. CD31 is valuable for researchers focusing on vascular biology and immunology serving as a beacon for cells involved in forming blood vessels or those located within the vascular system.

Biological function summary

CD31 is important for the transendothelial migration of leukocytes. It forms complex interactions with itself (homophilic binding) and other partners to mediate processes necessary for immune response and tissue integrity. This protein supports the development and maintenance of endothelial cell junctions making it fundamental in maintaining vascular permeability. Additionally CD31 plays a role in transmitting signals that influence the survival of leukocytes and endothelial cells. Its expression and function help regulate the balance between cell life and death impacting immune function and tissue repair.

Pathways

CD31 participates actively in both immune and angiogenesis pathways. It associates with proteins like SHP-2 and SHP-1 which are involved in downstream signaling cascades critical for cell signaling and immune cell activation. Within the angiogenesis pathway CD31 interacts with other adhesion molecules to promote new blood vessel formation under physiological and pathological conditions. It also collaborates with integrins influencing cellular responses to external stimuli and contributing to vascular remodeling and repair.

Associated diseases and disorders

CD31 is significantly associated with inflammatory conditions and cardiovascular diseases. For instance its dysfunction or altered expression can contribute to atherosclerosis a disease characterized by the hardening of arteries due to plaque buildup. Furthermore CD31 deficiencies have links with autoimmune disorders where it might affect leukocyte migration and contribute to tissue damage. The protein associates with ICAM-1 during inflammatory processes illustrating its complex involvement in disease progression and potential as a therapeutic target.

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9 product images

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] (ab182981)
Fluorescence multiplex immunohistochemical analysis of the human endometrium (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] ab223582, magenta; Opal™690), anti-NKG2A (Anti-NKG2A antibody [EPR23737-127] ab260035, green; Opal™520) and anti-CD31 (ab182981, red; Opal™570) on human endometrium. Panel B: anti-NKG2A stained on NK cells. Panel C: anti-CD31 stained on endothelial cells. Panel D: anti-EpCAM stained on glandular cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of Anti-EpCAM antibody [EPR20532-225] ab223582 at 1/500 dilution (1.008 μg/ml), Anti-NKG2A antibody [EPR23737-127] ab260035 at 1/2000 dilution (0.262 μg/ml) and ab182981 at 1/4000 dilution (0.137 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] (ab182981)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of rat lung tissue using rabbit Anti-CD31 antibody
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling CD31 with ab182981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in rat lung (PMID: 16234507) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] (ab182981)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of mouse lung tissue using rabbit Anti-CD31 antibody
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD31 with ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in mouse lung (PMID: 16234507) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] (ab182981)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human lung tissue using rabbit Anti-CD31 antibody
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling CD31 with ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in human lung (PMID: 16234507) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] (ab182981)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human kidney tissue using rabbit Anti-CD31 antibody
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD31 with ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in human kidney (PMID: 16234507) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] (ab182981)
CD31 Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-CD31 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696 at a 1/5000 dilution, ab182981 anti-CD31 used at 1/5000 dilution and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.

Panel B: anti-ALDH1L1 staining hepatocytes in mouse liver.

Panel C: anti-CD31 staining endothelium in mouse liver.

Panel D: anti-ABCB11 staining bile canaliculi in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696, ab182981 and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] (ab182981)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human lung using rabbit Anti-CD31 antibody
Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling CD31 with ab182981 at a concentration of 0.52 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab182981Anti-CD31 antibody was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] (ab182981)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human lung using rabbit Anti-CD31 antibody
Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling CD31 with ab182981 at a concentration of 0.35 µg/ml. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.ab182981Anti-CD31 antibody was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.
Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] (ab182981)
CD31 Multiplex immunohistochemistry staining of Rat liver using rabbit Anti-CD31 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696 at a 1/5000 dilution, ab182981 anti-CD31 used at 1/5000 dilution and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.

Panel B: anti-ALDH1L1 staining hepatocytes in rat liver.

Panel C: anti-CD31 staining endothelium in rat liver.

Panel D: anti-ABCB11 staining bile canaliculi in rat liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696, ab182981 and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Anti-CD31 antibody [EPR17259]

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