Anti-CD31 antibody [EPR17259] - BSA and Azide free

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

Fluorescence multiplex immunohistochemical analysis of the human endometrium (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-EpCAM (ab223582, magenta; Opal™690), anti-NKG2A (ab260035, green; Opal™520) and anti-CD31 (ab182981, red; Opal™570) on human endometrium. Panel B : anti-NKG2A stained on NK cells. Panel C : anti-CD31 stained on endothelial cells. Panel D : anti-EpCAM stained on glandular cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab223582 at 1/500 dilution (1.008 μg/ml), ab260035 at 1/2000 dilution (0.262 μg/ml) and ab182981 at 1/4000 dilution (0.137 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182981).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling CD31 with ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in human lung (PMID : 16234507) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182981).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD31 with ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in human kidney (PMID : 16234507) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182981).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (perfused fixed) tissue labeling CD31 with ab182981 at 1/200 (2.64 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing positive staining on mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with the primary antibody for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD31 with ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in mouse lung (PMID : 16234507) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182981).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling CD31 with ab182981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in rat lung (PMID : 16234507). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182981).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue staining PLGF with ab322267 at a 1/2000 dilution, ab324045 anti-AP2 gamma/TFAP2C used at 1/500 dilution and ab182981 anti-CD31 used at a 1/5000 dilution.

Panel A : merged staining of anti-PLGF (green; Opal™520), anti-AP2 gamma/TFAP2C (magenta; Opal™690) and anti-CD31 (gray; Opal™570) on mouse placenta.

Panel B : anti-PLGF showed positive staining in mouse placenta.

Panel C : anti-AP2 gamma/TFAP2C staining trophoblast stem cells in mouse placenta.

Panel D : anti-CD31 staining endothelium in mouse placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322267, ab324045 and ab182981 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue staining LOX 1 with ab317689 at a 1/2000 dilution, ab324045 anti-AP2 gamma used at 1/500 dilution and ab182981 anti-CD31 used at a 1/5000 dilution.

Panel A : merged staining of anti-LOX 1 (green; Opal™520), anti-AP2 gamma (magenta; Opal™690) and anti-CD31 (gray; Opal™570) on mouse placenta.

Panel B : anti-LOX 1 staining trophoblast in mouse placenta.

Panel C : ant-AP2 gamma staining trophoblast stem cell in mouse placenta.

Panel D : ant-CD31 staining endothelium in mouse placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317689, ab324045 and ab182981 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue staining LOX 1 with ab317689 at a 1/2000 dilution, ab324045 anti-AP2 gamma used at 1/500 dilution and ab182981 anti-CD31 used at a 1/5000 dilution.

Panel A : merged staining of anti-LOX 1 (green; Opal™520), anti-AP2 gamma (magenta; Opal™690) and anti-CD31 (gray; Opal™570) on mouse placenta.

Panel B : anti-LOX 1 staining trophoblast in mouse placenta.

Panel C : ant-AP2 gamma staining trophoblast stem cell in mouse placenta.

Panel D : ant-CD31 staining endothelium in mouse placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317689, ab324045 and ab182981 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab182981 anti-CD31 used at 1/5000 dilution and ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.
Panel B : anti-ALDH1L1 staining hepatocytes in mouse liver.
Panel C : anti-CD31 staining endothelium in mouse liver.
Panel D : anti-ABCB11 staining bile canaliculi in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab182981 and ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (perfused fixed) tissue labeling CD31 with ab182981 at 1/200 (2.64 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Low expression : confocal image showing weak staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with the primary antibody for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab182981 anti-CD31 used at 1/5000 dilution and ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.
Panel B : anti-ALDH1L1 staining hepatocytes in rat liver.
Panel C : anti-CD31 staining endothelium in rat liver.
Panel D : anti-ABCB11 staining bile canaliculi in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab182981 and ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (perfused fixed) tissue labeling CD31 with ab182981 at 1/200 (2.64 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Low expression : confocal image showing weak staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with the primary antibody for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883)

This data was developed using ab182981, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (perfused fixed) tissue labeling CD31 with ab182981 at 1/200 (2.64 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing positive staining on rat lung. The nuclear counterstain was DAPI (Blue). The section was incubated with the primary antibody for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).