Name: Anti-CD31 antibody [EPR17259] - BSA and Azide free
SKU: ab225883
Rating: 5 (2 reviews)

Anti-CD31 [EPR17259] antibody (ab225883) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect CD31 in IHC-P, mIHC. Suitable for Human, Mouse, Rat samples.

- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (AB225883), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IHC-P
Human	Tested	Tested
Mouse	Tested	Tested
Rat	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes In our hands we observed non-specific cytoplasmic staining on tubular cells in rat kidney. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes In our hands we observed non-specific cytoplasmic staining on tubular cells in rat kidney. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes In our hands we observed non-specific cytoplasmic staining on tubular cells in rat kidney. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Target data

See full target information PECAM1

Function

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.

Alternative names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR17259
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

What is this antibody validated in?

Anti-CD31 antibody [EPR17259] - BSA and Azide free (ab225883) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

Other related products

We have a range of other formats of antibody clone [EPR17259] also available for your convenience:
ab182981, Carrier free - ab225883, Alexa Fluor® 647 - ab305210, Alexa Fluor® 488 - ab305267

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (ab225883)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of rat lung tissue using rabbit Anti-CD31 antibody
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling CD31 with Anti-CD31 antibody [EPR17259] ab182981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in rat lung (PMID: 16234507). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [EPR17259] ab182981).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (ab225883)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of mouse lung tissue using rabbit Anti-CD31 antibody
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD31 with Anti-CD31 antibody [EPR17259] ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in mouse lung (PMID: 16234507) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [EPR17259] ab182981).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (ab225883)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human lung tissue using rabbit Anti-CD31 antibody
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling CD31 with Anti-CD31 antibody [EPR17259] ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in human lung (PMID: 16234507) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [EPR17259] ab182981).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR17259] - BSA and Azide free (ab225883)
CD31 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human kidney tissue using rabbit Anti-CD31 antibody
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD31 with Anti-CD31 antibody [EPR17259] ab182981 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on endothelial cells in human kidney (PMID: 16234507) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [EPR17259] ab182981).
Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (ab225883)
CD31 Multiplex immunohistochemistry staining of Rat liver using rabbit Anti-CD31 antibody
This data was developed using Anti-CD31 antibody [EPR17259] ab182981, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696 at a 1/5000 dilution, Anti-CD31 antibody [EPR17259] ab182981 anti-CD31 used at 1/5000 dilution and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.

Panel B: anti-ALDH1L1 staining hepatocytes in rat liver.

Panel C: anti-CD31 staining endothelium in rat liver.

Panel D: anti-ABCB11 staining bile canaliculi in rat liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696, Anti-CD31 antibody [EPR17259] ab182981 and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (ab225883)
CD31 Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-CD31 antibody
This data was developed using Anti-CD31 antibody [EPR17259] ab182981, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696 at a 1/5000 dilution, Anti-CD31 antibody [EPR17259] ab182981 anti-CD31 used at 1/5000 dilution and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 anti-ABCB11 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-ALDH1L1 (green; Opal™520), anti-CD31 (magenta; Opal™690) and anti-ABCB11 (grey; Opal™570) on mouse liver.

Panel B: anti-ALDH1L1 staining hepatocytes in mouse liver.

Panel C: anti-CD31 staining endothelium in mouse liver.

Panel D: anti-ABCB11 staining bile canaliculi in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker ab307696, Anti-CD31 antibody [EPR17259] ab182981 and Anti-ABCB11/BSEP antibody [EPR28773-83] ab315474 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] - BSA and Azide free (ab225883)
Fluorescence multiplex immunohistochemical analysis of the human endometrium (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] ab223582, magenta; Opal™690), anti-NKG2A (Anti-NKG2A antibody [EPR23737-127] ab260035, green; Opal™520) and anti-CD31 (Anti-CD31 antibody [EPR17259] ab182981, red; Opal™570) on human endometrium. Panel B: anti-NKG2A stained on NK cells. Panel C: anti-CD31 stained on endothelial cells. Panel D: anti-EpCAM stained on glandular cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of Anti-EpCAM antibody [EPR20532-225] ab223582 at 1/500 dilution (1.008 μg/ml), Anti-NKG2A antibody [EPR23737-127] ab260035 at 1/2000 dilution (0.262 μg/ml) and Anti-CD31 antibody [EPR17259] ab182981 at 1/4000 dilution (0.137 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [EPR17259] ab182981).

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Anti-CD31 antibody [EPR17259] - BSA and Azide free

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