Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB, sELISA and reacts with Mouse, Rat samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | sELISA | Flow Cyt | |
---|---|---|---|---|
Mouse | Tested | Tested | Tested | Not recommended |
Rat | Expected | Tested | Expected | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/40 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Species Rat | Dilution info 1/1000 | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Not suitable for FC-Mouse. |
Species Rat | Dilution info - | Notes - |
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Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).
Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1
Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB, sELISA and reacts with Mouse, Rat samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR17260-254
Affinity purification Protein A
Not suitable for FC-Mouse.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.
For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).
For a complete ELISA kit, see Mouse CD31 ELISA kit (Mouse CD31 ELISA Kit (PECAM1) ab204527).
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/20000 dilution
Lane 2: Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/100000 dilution
All lanes: Mouse CD31 active protein at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 100 kDa
Exposure time: 3min
CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: bEnd.3 whole cell lysate 10μg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab213175 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (ab213175)
Predicted band size: 82 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-3: 10 seconds; Lane 4/5: 1 second.
The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).
Lanes 1 - 4: Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/1000 dilution
Lane 5: Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/2000 dilution
Lane 1: Mouse platelet lysate at 10 µg
Lane 2: Rat platelet lysate at 10 µg
Lane 3: Rat lung lysate at 10 µg
Lane 4: Mouse lung lysate at 10 µg
Lane 5: bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 82 kDa
Observed band size: 110-130 kDa
Anti-CD31 ab213175 was used with Tissue clearing kit – CUBIC (Tissue Clearing Kit – CUBIC ab316246) and 3D Tissue Staining Kit – CUBIC (3D Tissue Staining Kit – CUBIC ab316248) to penetrate, stain and clear a whole mouse brain.
Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample.
For a whole mouse brain, we recommend starting with 10 ug of ab213175 and using a Fab fragment secondary antibody with 6.67 µg to create an antibody complex before 3D staining (see protocol for details). No additive was used during the staining process.
The sample was imaged using a light-sheet microscopy.
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