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Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB, sELISA and reacts with Mouse, Rat samples. Cited in 2 publications.

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Images

ELISA - Anti-CD31 antibody [EPR17260-254] (AB213175), expandable thumbnail
  • Western blot - Anti-CD31 antibody [EPR17260-254] (AB213175), expandable thumbnail
  • Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (AB213175), expandable thumbnail
  • Western blot - Anti-CD31 antibody [EPR17260-254] (AB213175), expandable thumbnail
  • IHC-tissue clearing - Anti-CD31 antibody [EPR17260-254] (AB213175), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBsELISAFlow Cyt
Mouse
Tested
Tested
Tested
Not recommended
Rat
Expected
Tested
Expected
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/40

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Ms Isoform 1-4: 69.8-81.3 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).

Species

Rat

Dilution info

1/1000

Notes

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Ms Isoform 1-4: 69.8-81.3 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Not suitable for FC-Mouse.

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

1 product for Alternative Version

Target data

Function

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB, sELISA and reacts with Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17260-254

Purification technique

Affinity purification Protein A

Specificity

Not suitable for FC-Mouse.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • ELISA - Anti-CD31 antibody [EPR17260-254] (ab213175), expandable thumbnail

    ELISA - Anti-CD31 antibody [EPR17260-254] (ab213175)

    Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.

    For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).

    For a complete ELISA kit, see Mouse CD31 ELISA kit (Mouse CD31 ELISA Kit (PECAM1) ab204527).

  • Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175), expandable thumbnail

    Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lane 1: Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/20000 dilution

    Lane 2: Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/100000 dilution

    All lanes: Mouse CD31 active protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 82 kDa

    Observed band size: 100 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (ab213175), expandable thumbnail

    Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (ab213175)

    CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution

    Lane 1: bEnd.3 whole cell lysate 10μg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab213175 in bEnd.3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (ab213175)

    Predicted band size: 82 kDa

  • Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175), expandable thumbnail

    Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-3: 10 seconds; Lane 4/5: 1 second.

    The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).

    Lanes 1 - 4: Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/1000 dilution

    Lane 5: Western blot - Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/2000 dilution

    Lane 1: Mouse platelet lysate at 10 µg

    Lane 2: Rat platelet lysate at 10 µg

    Lane 3: Rat lung lysate at 10 µg

    Lane 4: Mouse lung lysate at 10 µg

    Lane 5: bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 82 kDa

    Observed band size: 110-130 kDa

  • IHC-tissue clearing - Anti-CD31 antibody [EPR17260-254] (ab213175), expandable thumbnail

    IHC-tissue clearing - Anti-CD31 antibody [EPR17260-254] (ab213175)

    Anti-CD31 ab213175 was used with Tissue clearing kit – CUBIC (Tissue Clearing Kit – CUBIC ab316246) and 3D Tissue Staining Kit – CUBIC (3D Tissue Staining Kit – CUBIC ab316248) to penetrate, stain and clear a whole mouse brain.

    Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample.

    For a whole mouse brain, we recommend starting with 10 ug of ab213175 and using a Fab fragment secondary antibody with 6.67 µg  to create an antibody complex before 3D staining (see protocol for details). No additive was used during the staining process.

    The sample was imaged using a light-sheet microscopy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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