Rabbit Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for IP, WB, sELISA and reacts with Mouse, Rat samples.
Images
![ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (AB224260), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-254-low-endotoxin-azide-free-ab224260--elisa-img76134.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (AB224260)")
![Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (AB224260), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-254-low-endotoxin-azide-free-ab224260--immunoprecipitation-img21449.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (AB224260)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | sELISA | |
|---|---|---|---|---|
| Mouse | Tested | Not recommended | Tested | Tested |
| Rat | Expected | Not recommended | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Not suitable for FC-Mouse. |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Species Rat | Dilution info - | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
7 products for Alternative Product
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).
Alternative names
CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1
Recommended products
Rabbit Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for IP, WB, sELISA and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17260-254
- Purification technique
- Affinity purification Protein A
- Specificity
Not suitable for FC-Mouse.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab224260 is the carrier-free version of Anti-CD31 antibody [EPR17260-254] ab213175.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-254-low-endotoxin-azide-free-ab224260--elisa-img76134.jpg "ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)")
ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
This ELISA data was generated using the same anti-CD31 antibody clone [EPR17260-254] in a different buffer formulation (cat# Anti-CD31 antibody [EPR17260-254] ab213175).
Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (Anti-CD31 antibody [EPR17260-254] ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.
For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).
For a complete ELISA kit, see Mouse CD31 ELISA kit (Mouse CD31 ELISA Kit (PECAM1) ab204527).
![Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-254-low-endotoxin-azide-free-ab224260--immunoprecipitation-img21449.jpg "Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)")
Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with Anti-CD31 antibody [EPR17260-254] ab213175 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using Anti-CD31 antibody [EPR17260-254] ab213175 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: bEnd.3 whole cell lysate 10μg (Input). Lane 2: Anti-CD31 antibody [EPR17260-254] ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD31 antibody [EPR17260-254] ab213175 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [EPR17260-254] ab213175).
All lanes: Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (Anti-CD31 antibody [EPR17260-254] ab213175)
Predicted band size: 82 kDa
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