Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free
- RabMAb
- Recombinant
- Advanced Validation
- What is this?
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(1 Publication)
Rabbit Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for IP, WB, sELISA, IHC - Wmt and reacts with Mouse, Rat samples. Cited in 1 publication.
View Alternative Names
CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1
- IP
Supplier Data
Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (AB224260)
CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : bEnd.3 whole cell lysate 10μg (Input). Lane 2 : ab213175 IP in bEnd.3 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213175 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213175).
All lanes:
Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] (<a href='/en-us/products/primary-antibodies/cd31-antibody-epr17260-254-ab213175'>ab213175</a>)
Predicted band size: 82 kDa
false
- ELISA
Supplier Data
ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (AB224260)
This ELISA data was generated using the same anti-CD31 antibody clone [EPR17260-254] in a different buffer formulation (cat# ab213175).
Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.
For a matched antibody pair set see Mouse CD31 Matched Antibody Pair Kit (ab212065).
For a complete ELISA kit see Mouse CD31 ELISA kit (ab204527).
Related conjugates and formulations (3)
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Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (Detector)
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Anti-CD31 antibody [EPR17260-254]
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Anti-CD31 antibody [EPR17260-254] - BSA and Azide free
Reactivity data
Product details
ab224260 is the carrier-free version of ab213175.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 14:6446 PubMed37833281
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com