Anti-CD31 antibody [EPR17260-263]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (AB222783)

Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CD31 with ab222783 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on bEND.3 cells. Negative control : NIH/3T3 (PMID : 1429859).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• WB

Supplier Data

Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783)

Exposure time : Lane 1-2 : 3 minutes; Lane 3 : 5 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 10448867).

All lanes:

Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution

Lane 1:

Mouse platelet lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

Mouse lung lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 110-130 kDa

true

• WB

Supplier Data

Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : NIH/3T3 (PMID : 1429859).

All lanes:

Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution

Lane 1:

bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 110 kDa

true

Exposure time: 3s

• WB

CiteAb

Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783)

CD31 western blot using anti-CD31 antibody [EPR17260-263] ab222783. Publication image and figure legend from Zhang, C., Luo, D., et al., 2019, Stem Cells Int, PubMed 31582984.

ab222783 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab222783 please see the product overview.

USCs improved erectile function and cavernosal endothelial function in diabetic erectile dysfunction (DED) rats. (a) Representative ICP tracing response to the stimulation of cavernous nerve (2 ms, 5 V, 25 Hz, and duration of 60 s) in normal rats and DED rats 4 weeks after intracavernous injection of PBS or USCs. (b) The USC injection increased ICP of DED rats, and (c) the ratio of ICP to MAP was calculated for each group (n = 8 per group). (d) Western blot and (e) quantification for western blot revealed that CD31, eNOS, p-eNOS, VEGFRA, and VEGFR2 expressions were all increased in the USC-treated group 4 weeks after cell transplantation (n = 6 per group). (f) Immunofluorescent staining confirmed the significantly higher expression of CD31 in cavernous tissue. *p < 0.05. ICP : intracavernous pressure; MAP : mean arterial pressure; p-eNOS : phospho-eNOS.

false