Anti-CD31 antibody [EPR17260-263] (ab222783) is a rabbit monoclonal antibody detecting CD31 in Western Blot, ICC/IF. Suitable for Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (AB222783), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-263-ab222783--immunocytochemistry-immunofluorescence-img170477.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (AB222783)")
![Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-263-ab222783--western-blot-img159005.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783)")
![Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-263-ab222783--western-blot-img150940.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783)")
![Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-263-ab222783--western-blot-img442752.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783)")
Publications
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- JCI insight 9:2024Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPu-Hong Zhang,Wen-Wu Zhang,Shun-Shun Wang,Cheng-Hua Wu,Yang-Dong Ding,Xin-Yi Wu,Fang Gao Smith,Yu Hao,Sheng-Wei JinPubMed 37971881
- Journal of translational medicine 21:8202023YAP1/Piezo1 involve in the dynamic changes of lymphatic vessels in UVR-induced photoaging progress to squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuling L Yang,Chu Zhou,Qi Chen,Shuzhan Z Shen,Jiandan D Li,Xiuli L Wang,Peiru R WangPubMed 37974224
- Diabetology & metabolic syndrome 15:2342023Human umbilical cord mesenchymal stem cell-derived exosomes promote microcirculation in aged diabetic mice by TGF-β1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeijian Fan,Mengdie Zhou,Shaoqiu Zheng,Yang Liu,Songsong Pan,Peng Guo,Minjie Xu,Chao Hu,Anle Ding,Zan Wang,Shiwu Yin,Keqiang Zuo,Xiaoyun XiePubMed 37968711
- Drug design, development and therapy 17:3249-32672023L. and Bunge. Enhances Angiogenesis by Regulating the miR-155-5p/HIF-1α/VEGF Axis in Acute Myocardial Infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingxing Li,Rongpeng Liu,Wei Liu,Xin Liu,Zongjing Fan,Jie Cui,Yang Wu,Huijun Yin,Quan LinPubMed 37954484
- JCI insight 8:2023Signaling metabolite succinylacetone activates HIF-1α and promotes angiogenesis in GSTZ1-deficient hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuating Luo,Qiujie Wang,Fan Yang,Rui Liu,Qingzhu Gao,Bin Cheng,Xue Lin,Luyi Huang,Chang Chen,Jin Xiang,Kai Wang,Bo Qin,Ni TangPubMed 37906252
- iScience 26:1082702023Fucoxanthin ameliorates traumatic brain injury by suppressing the blood-brain barrier disruption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Zhang,Zhigang Hu,Wanshan Bai,Yaonan Peng,Yixing Lin,Zixiang CongPubMed 37965135
- Particle and fibre toxicology 20:362023Long-term PM2.5 exposure disrupts corneal epithelial homeostasis by impairing limbal stem/progenitor cells in humans and rat models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengjie Hao,Zhijian Chen,Yuzhou Gu,Lu Chen,Feiyin Sheng,Yili Xu,Di Wu,Yu Han,Bing Lu,Shuying Chen,Wei Zhao,Houfa Yin,Xiaofeng Wang,S Amer Riazuddin,Xiaoming Lou,Qiuli Fu,Ke YaoPubMed 37759270
- Materials today. Bio 23:1008092023Combination of irinotecan silicasome nanoparticles with radiation therapy sensitizes immunotherapy by modulating the activation of the cGAS/STING pathway for colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Wang,Tianyu Zhang,Yile Zheng,Yuting Li,Xiyuan Tang,Qianping Chen,Wei Mao,Weiwei Li,Xiangsheng Liu,Ji ZhuPubMed 37779919
- iScience 26:1080102023Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakeshi Kameyama,Muneaki Miyata,Hajime Shiotani,Jun Adachi,Soichiro Kakuta,Yasuo Uchiyama,Kiyohito Mizutani,Yoshimi TakaiPubMed 37829206
- JACS Au 3:2597-26082023DNA-Based Hydrogels with Multidrug Sequential Release for Promoting Diabetic Wound Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Li,Hui Xie,Liping Gou,Ye Zhou,Hao Wang,Ruoqing Li,Yong Zhang,Shuyun Liu,Jingping Liu,Yanrong Lu,Zhengliang Eric He,Nan Chen,Jiang Li,Ying Zhu,Chengshi Wang,Min LvPubMed 37772175
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes In WB, under our testing conditions, we observe an additional band (~50 kDa) for some human, mouse and rat cell and tissue lysates. Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Species Rat | Dilution info 1/2000 | Notes In WB, under our testing conditions, we observe an additional band (~50 kDa) for some human, mouse and rat cell and tissue lysates. Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Associated Products
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Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).
Alternative names
CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1
Recommended products
Anti-CD31 antibody [EPR17260-263] (ab222783) is a rabbit monoclonal antibody detecting CD31 in Western Blot, ICC/IF. Suitable for Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR17260-263
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CD31 antibody [EPR17260-263] (ab222783) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, WB in mouse, rat samples.
Anti-CD31 antibody [EPR17260-263] (ab222783) has been cited over 72 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD31 antibody [EPR17260-263] (ab222783) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD31 antibody [EPR17260-263] (ab222783) has 11 independent reviews from customers.
Anti-CD31 antibody [EPR17260-263] (ab222783) specifically detects CD31 (UniProt ID: Q08481; Molecular weight: 79kDa) and is sold in 100 µL and 1 mL selling sizes.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-263-ab222783--immunocytochemistry-immunofluorescence-img170477.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)")
Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)
Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CD31 with ab222783 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on bEND.3 cells. Negative control: NIH/3T3 (PMID: 1429859).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-263-ab222783--western-blot-img159005.jpg "Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)")
Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)
Blocking/Dilution buffer: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID: 1429859).
All lanes: Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution
Lane 1: bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 110 kDa
Exposure time: 3s
![Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-263-ab222783--western-blot-img150940.jpg "Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)")
Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)
Exposure time : Lane 1-2: 3 minutes; Lane 3: 5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 10448867).
All lanes: Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution
Lane 1: Mouse platelet lysate at 20 µg
Lane 2: Rat lung lysate at 20 µg
Lane 3: Mouse lung lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 110-130 kDa
![Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-epr17260-263-ab222783--western-blot-img442752.jpg "Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)
CD31 western blot using anti-CD31 antibody [EPR17260-263] ab222783. Publication image and figure legend from Zhang, C., Luo, D., et al., 2019, Stem Cells Int, PubMed 31582984.
ab222783 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab222783 please see the product overview.
USCs improved erectile function and cavernosal endothelial function in diabetic erectile dysfunction (DED) rats. (a) Representative ICP tracing response to the stimulation of cavernous nerve (2 ms, 5 V, 25 Hz, and duration of 60 s) in normal rats and DED rats 4 weeks after intracavernous injection of PBS or USCs. (b) The USC injection increased ICP of DED rats, and (c) the ratio of ICP to MAP was calculated for each group (n = 8 per group). (d) Western blot and (e) quantification for western blot revealed that CD31, eNOS, p-eNOS, VEGFRA, and VEGFR2 expressions were all increased in the USC-treated group 4 weeks after cell transplantation (n = 6 per group). (f) Immunofluorescent staining confirmed the significantly higher expression of CD31 in cavernous tissue. ∗p < 0.05. ICP: intracavernous pressure; MAP: mean arterial pressure; p-eNOS: phospho-eNOS.
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