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Rabbit Recombinant Monoclonal CD31 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat samples. Cited in 72 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (AB222783), expandable thumbnail
  • Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783), expandable thumbnail
  • Western blot - Anti-CD31 antibody [EPR17260-263] (AB222783), expandable thumbnail

Publications

  • JCI insight 9:2024
    Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Pu-Hong Zhang,Wen-Wu Zhang,Shun-Shun Wang,Cheng-Hua Wu,Yang-Dong Ding,Xin-Yi Wu,Fang Gao Smith,Yu Hao,Sheng-Wei Jin
    PubMed 37971881
  • Journal of translational medicine 21:8202023
    YAP1/Piezo1 involve in the dynamic changes of lymphatic vessels in UVR-induced photoaging progress to squamous cell carcinoma.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yuling L Yang,Chu Zhou,Qi Chen,Shuzhan Z Shen,Jiandan D Li,Xiuli L Wang,Peiru R Wang
    PubMed 37974224

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Use 0.3M glycine to quench autofluorescence caused by aldehydes.

Positive Control: HUVEC cells

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

In WB, under our testing conditions, we observe an additional band (~50 kDa) for some human, mouse and rat cell and tissue lysates.

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Ms Isoform 1-4: 69.8-81.3 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).

Species

Rat

Dilution info

1/2000

Notes

In WB, under our testing conditions, we observe an additional band (~50 kDa) for some human, mouse and rat cell and tissue lysates.

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Ms Isoform 1-4: 69.8-81.3 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).

Associated Products

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      Target data

      Function

      Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).

      Alternative names

      Platelet endothelial cell adhesion molecule, PECAM-1, Pecam-1, Pecam1, Pecam

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      Rabbit Recombinant Monoclonal CD31 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat samples. Cited in 72 publications.

      Alternative names

      Platelet endothelial cell adhesion molecule, PECAM-1, Pecam-1, Pecam1, Pecam

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR17260-263

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      3 product images

      • Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CD31 with ab222783 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on bEND.3 cells. Negative control: NIH/3T3 (PMID: 1429859).

        The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

      • Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783), expandable thumbnail

        Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        Negative control: NIH/3T3 (PMID: 1429859).

        All lanes: Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution

        Lane 1: bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg

        Lane 2: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Developed using the ECL technique.

        Predicted band size: 82 kDa

        Observed band size: 110 kDa

        Exposure time: 3s

      • Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783), expandable thumbnail

        Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)

        Exposure time : Lane 1-2: 3 minutes; Lane 3: 5 seconds.

        Blocking/Dilution buffer: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 10448867).

        All lanes: Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution

        Lane 1: Mouse platelet lysate at 20 µg

        Lane 2: Rat lung lysate at 20 µg

        Lane 3: Mouse lung lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Developed using the ECL technique.

        Predicted band size: 82 kDa

        Observed band size: 110-130 kDa

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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