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Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt
Mouse
Tested
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/40

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Ms Isoform 1-4: 69.8-81.3 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).

Alternative names

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Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17260-265

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-CD31 antibody [EPR17260-265] (ab182982), expandable thumbnail

    Western blot - Anti-CD31 antibody [EPR17260-265] (ab182982)

    Lane 1: Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982) at 1/20000 dilution

    Lane 2: Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982) at 1/100000 dilution

    All lanes: His-tagged mouse CD31 recombinant protein (aa18-590) 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 82 kDa

    Observed band size: 100 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] (ab182982), expandable thumbnail

    Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] (ab182982)

    CD31 was immunoprecipitated from 1 mg bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab182982 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182982 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: bEnd.3 whole cell lysate 10 μg (Input).
    Lane 2: ab182982 IP in bEnd.3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182982 in bEnd.3 whole cell lysate (-)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] (AB182982)

    Predicted band size: 82 kDa

    Observed band size: 110-130 kDa

    Exposure time: 3s

  • Western blot - Anti-CD31 antibody [EPR17260-265] (ab182982), expandable thumbnail

    Western blot - Anti-CD31 antibody [EPR17260-265] (ab182982)

    Blocking: 5% NFDM /TBST.

    Exposure time:

    Lane 1: 3 minutes

    Lanes 2-3: 1 second

    The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).

    Lanes 1 - 2: Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982) at 1/1000 dilution

    Lane 3: Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982) at 1/2000 dilution

    Lane 1: Mouse platelet lysate at 10 µg

    Lane 2: Mouse lung lysate at 10 µg

    Lane 3: bEnd.3 (mouse brain endothelioma) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 82 kDa

    Observed band size: 110-130 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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