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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | Flow Cyt | |
---|---|---|---|
Mouse | Tested | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/40 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).
Platelet endothelial cell adhesion molecule, PECAM-1, Pecam-1, Pecam1, Pecam
Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications.
Platelet endothelial cell adhesion molecule, PECAM-1, Pecam-1, Pecam1, Pecam
IgG
Rabbit
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR17260-265
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Lane 1: Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982) at 1/20000 dilution
Lane 2: Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982) at 1/100000 dilution
All lanes: His-tagged mouse CD31 recombinant protein (aa18-590) 10 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 100 kDa
Exposure time: 3min
CD31 was immunoprecipitated from 1 mg bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab182982 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182982 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: bEnd.3 whole cell lysate 10 μg (Input).
Lane 2: ab182982 IP in bEnd.3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182982 in bEnd.3 whole cell lysate (-)
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] (AB182982)
Predicted band size: 82 kDa
Observed band size: 110-130 kDa
Exposure time: 3s
Blocking: 5% NFDM /TBST.
Exposure time:
Lane 1: 3 minutes
Lanes 2-3: 1 second
The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).
Lanes 1 - 2: Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982) at 1/1000 dilution
Lane 3: Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982) at 1/2000 dilution
Lane 1: Mouse platelet lysate at 10 µg
Lane 2: Mouse lung lysate at 10 µg
Lane 3: bEnd.3 (mouse brain endothelioma) whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 110-130 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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