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AB182982

Anti-CD31 antibody [EPR17260-265]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal CD31 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 5 publications.

View Alternative Names

CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1

3 Images
Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] (AB182982)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] (AB182982)

CD31 was immunoprecipitated from 1 mg bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab182982 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182982 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : bEnd.3 whole cell lysate 10 μg (Input).
Lane 2 : ab182982 IP in bEnd.3 whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab182982 in bEnd.3 whole cell lysate (-)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] (ab182982)

Predicted band size: 82 kDa

Observed band size: 110-130 kDa

false

Exposure time: 3s

Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982)
  • WB

Supplier Data

Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982)

Blocking : 5% NFDM /TBST.

Exposure time :

Lane 1 : 3 minutes

Lanes 2-3 : 1 second

The expression MW observed is consistent with what has been described in the literature (PMID : 18388311; 12433657).

Lanes 1 - 2:

Western blot - Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/1000 dilution

Lane 3:

Western blot - Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/2000 dilution

Lane 1:

Mouse platelet lysate at 10 µg

Lane 2:

Mouse lung lysate at 10 µg

Lane 3:

bEnd.3 (mouse brain endothelioma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 110-130 kDa

true

Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982)
  • WB

Supplier Data

Western blot - Anti-CD31 antibody [EPR17260-265] (AB182982)

Lane 1:

Western blot - Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/20000 dilution

Lane 2:

Western blot - Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/100000 dilution

All lanes:

His-tagged mouse CD31 recombinant protein (aa18-590) 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 82 kDa

Observed band size: 100 kDa

true

Exposure time: 3min

  • Carrier free

    Anti-CD31 antibody [EPR17260-265] - BSA and Azide free

  • Carrier free

    Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (Capture)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17260-265

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.</p><p>The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.</p><p>Ms Isoform 1-4: 69.8-81.3 kDa (predicted).</p><p>Observed band size is around 130 kDa.</p><p>Positive Control: HUVEC and Jurkat cell lysates (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>); Human spleen and kidney tissue lysate.</p><p>Negative Control: NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>).</p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed : 19048083).
See full target information Pecam1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:706 PubMed41053124

2025

Cancer-associated fibroblasts expressing FSTL3 promote vasculogenic mimicry formation and drive colon cancer malignancy.

Applications

Unspecified application

Species

Unspecified reactive species

Leqian Ying,Yini Zhu,Lu Zhang,Min Ji,Meidan Wang,Lei Dong,Zhengcheng Yun,Yanping Chen,Jingyi Zhou,Chunchun Huang,Shengwei Zhang,Xuhong Yang,Hui Yang,Guichun Huang,Shukui Qin,Jinbing Xie,Lin Liu

Theranostics 15:9580-9600 PubMed41041054

2025

Macrophage-TREM2 promotes cardiac repair by restricting the infiltration of CD8 T cells via CXCL16-CXCR6 axis after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Zhang,Sheng Wang,Yu Ding,Tao Zheng,Jie Sheng,Yanshan Chen,Zhiyue Wang,Ximei Dai,Canbiao Wang,Long Ma,Jing Pan,Yunming Zhang,Longjiang Zhang

Cells 12: PubMed37887278

2023

Aurora Kinase A Regulates Cell Transitions in Glucocorticoid-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Qiao,Yang Yang,Yan Zhao,Xiuju Wu,Li Zhang,Xinjiang Cai,Jaden Ji,Kristina I Boström,Yucheng Yao

Cells 12: PubMed37508475

2023

Cell Transitions Contribute to Glucocorticoid-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Qiao,Xiuju Wu,Yan Zhao,Yang Yang,Li Zhang,Xinjiang Cai,Jocelyn A Ma,Jaden Ji,Karen Lyons,Kristina I Boström,Yucheng Yao

Molecular neurobiology 56:7631-7650 PubMed31089963

2019

Transcriptional Response and Morphological Features of the Neurovascular Unit and Associated Extracellular Matrix After Experimental Stroke in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Aleithe,Alexandra Blietz,Bianca Mages,Constance Hobusch,Wolfgang Härtig,Dominik Michalski
View all publications
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Product promise

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