Anti-CD31 antibody [EPR17260-265] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (AB232227)

CD31 was immunoprecipitated from 1 mg bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab182982 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182982 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : bEnd.3 whole cell lysate 10 μg (Input).
Lane 2 : ab182982 IP in bEnd.3 whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab182982 in bEnd.3 whole cell lysate (-)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182982).

All lanes:

Immunoprecipitation - Anti-CD31 antibody [EPR17260-265] (<a href='/en-us/products/primary-antibodies/cd31-antibody-epr17260-265-ab182982'>ab182982</a>)

Predicted band size: 82 kDa

Observed band size: 110-130 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-CD31 antibody [EPR17260-265] - BSA and Azide free (AB232227)

Blocking : 5% NFDM /TBST.

Exposure time : Lane 1 : 3 minutes; Lanes 2-3 : 1 second.

The expression MW observed is consistent with what has been described in the literature (PMID : 18388311; 12433657).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182982).

Lanes 1 - 2:

Western blot - Anti-CD31 antibody [EPR17260-265] (<a href='/en-us/products/primary-antibodies/cd31-antibody-epr17260-265-ab182982'>ab182982</a>) at 1/1000 dilution

Lane 3:

Western blot - Anti-CD31 antibody [EPR17260-265] (<a href='/en-us/products/primary-antibodies/cd31-antibody-epr17260-265-ab182982'>ab182982</a>) at 1/2000 dilution

Lane 1:

Mouse platelet lysate at 10 µg

Lane 2:

Mouse lung lysate at 10 µg

Lane 3:

bEnd.3 (mouse brain endothelioma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 82 kDa

Observed band size: 110-130 kDa

true