Mouse Monoclonal CD31 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Tissue preparation containing PECAM1 protein.
Images
![Flow Cytometry - Anti-CD31 antibody [JC/70A] - BSA and Azide free (AB212712), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-jc-70a-bsa-and-azide-free-ab212712--flow-cytometry-img133059.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD31 antibody [JC/70A] - BSA and Azide free (AB212712)")
![Western blot - Anti-CD31 antibody [JC/70A] - BSA and Azide free (AB212712), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-jc-70a-bsa-and-azide-free-ab212712--western-blot-img39940.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD31 antibody [JC/70A] - BSA and Azide free (AB212712)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (AB212712), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-jc-70a-bsa-and-azide-free-ab212712--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67803.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (AB212712)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (AB212712), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-jc-70a-bsa-and-azide-free-ab212712--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44419.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (AB212712)")
Publications
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- Acta orthopaedica et traumatologica turcica 56:178-1862022The effect of endostatin on angiogenesis and osteogenesis of steroid-induced osteonecrosis of the femoral head in a rabbit model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhao,Dong Li,Da-Peng Duan,Qi-Chun SongPubMed 35703505
- Biomedicines 10:2022Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFernando Gisbert Roca,Luis Gil Santos,Manuel Mata Roig,Lara Milian Medina,Cristina Martínez-Ramos,Manuel Monleón PradasPubMed 35625700
- Stem cell reports 17:307-3202022The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusanne Krasemann,Undine Haferkamp,Susanne Pfefferle,Marcel S Woo,Fabian Heinrich,Michaela Schweizer,Antje Appelt-Menzel,Alevtina Cubukova,Janica Barenberg,Jennifer Leu,Kristin Hartmann,Edda Thies,Jessica Lisa Littau,Diego Sepulveda-Falla,Liang Zhang,Kathy Ton,Yan Liang,Jakob Matschke,Franz Ricklefs,Thomas Sauvigny,Jan Sperhake,Antonia Fitzek,Anna Gerhartl,Andreas Brachner,Nina Geiger,Eva-Maria König,Jochen Bodem,Sören Franzenburg,Andre Franke,Stefan Moese,Franz-Josef Müller,Gerd Geisslinger,Carsten Claussen,Aimo Kannt,Andrea Zaliani,Philip Gribbon,Benjamin Ondruschka,Winfried Neuhaus,Manuel A Friese,Markus Glatzel,Ole PlessPubMed 35063125
- Artificial organs 45:1491-15002021Decellularized tracheal prelamination implant: A proposed bilateral double organ technique.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNéstor J Martínez-Hernández,Ana Díaz-Cuevas,Lara Milián-Medina,María Sancho-Tello,Joan Roselló-Ferrando,Alfonso Morcillo-Aixelá,José Luis Campo-Cañaveral,Amparo Roig-Bataller,Manuel Mata-RoigPubMed 34310703
- Science advances 6:2020Novel fractionated ultrashort thermal exposures with MRI-guided focused ultrasound for treating tumors with thermosensitive drugs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarc A Santos et. al.PubMed 32917589
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Tissue preparation containing PECAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P16284
Consider this alternative
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended |
| Cynomolgus monkey | Not recommended | Not recommended | Not recommended |
| Pig | Not recommended | Not recommended | Not recommended |
| Rabbit | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg for 106 Cells | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Pig | Dilution info - | Notes - |
Species Rabbit | Dilution info - | Notes - |
Species Cynomolgus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Hu Isoform 1-6: 79-83 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Hu Isoform 1-6: 79-83 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Species Pig | Dilution info - | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Hu Isoform 1-6: 79-83 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Species Rabbit | Dilution info - | Notes - |
Species Cynomolgus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Pig | Dilution info - | Notes The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rabbit | Dilution info - | Notes - |
Species Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.
Alternative names
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Mouse Monoclonal CD31 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Tissue preparation containing PECAM1 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Tissue preparation containing PECAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P16284
- Carrier free
- Yes
- Clone number
- JC/70A
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-jc-70a-bsa-and-azide-free-ab212712--flow-cytometry-img133059.jpg "Flow Cytometry - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)")
Flow Cytometry - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)
Flow Cytometry analysis of Jurkat cells labeling CD31 with ab212712 (Green). Black: cells alone; Grey: Isotype Control.
![Western blot - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-jc-70a-bsa-and-azide-free-ab212712--western-blot-img39940.jpg "Western blot - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)")
Western blot - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)
All lanes: Western blot - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)
All lanes: THP-1 cell lysate
Predicted band size: 82 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-jc-70a-bsa-and-azide-free-ab212712--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67803.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling CD31 with ab212712 at 2 μg/ml.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-jc-70a-bsa-and-azide-free-ab212712--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44419.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab212712)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human angiosarcoma tissue labeling CD31 with ab212712 at 2 μg/ml dilution.
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