JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB256569

Anti-CD31 antibody [MEC 13.3]

Be the first to review this product! Submit a review

|

(4 Publications)

Rat Recombinant Monoclonal CD31 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 13.3] (AB256569)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 13.3] (AB256569)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized b.end3 and B16-F0(-) cells labelling CD31 with ab256569 at 1/50 dilution (21.16μg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2μg/ml) (Green). Confocal image showing membranous and cytoplasmic staining in b.end3 cells.

Negative control : B16-F0 (PMID : 8082649).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5μg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4μg/ml) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab256569 at a 1/50 dilution (21.16μg/ml) followed by ab150080 at a 1/500 dilution (4μg/ml).

Negative control 2 : ab179513 at 1/200 dilution (2.5μg/ml) followed by ab150157 at a 1/1000 dilution (2μg/ml).

Flow Cytometry - Anti-CD31 antibody [MEC 13.3] (AB256569)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD31 antibody [MEC 13.3] (AB256569)

Flow cytometric analysis of B16-F0 (mouse melanoma epithelial cell-like) (Left panel) / bEnd.3 (mouse brain endothelioma) (Right panel) cells labelling CD31 with ab256569 at 1/1000 dilution (1.058μg/ml) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

Negative control : B16-F0 (PMID : 8082649).

Gated on viable cells.

  • Carrier free

    Anti-CD31 antibody [MEC 13.3] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MEC 13.3

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB204527

Mouse CD31 ELISA Kit (PECAM1)

Recombinant|SimpleStep

Sandwich ELISA - Mouse CD31 ELISA Kit (PECAM1) (AB204527)

  • 0
  • 0
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "21.16 µg/mL", "ICCIF-species-notes": "<p>Use 0.3M glycine to quench autofluorescence caused by aldehydes.</p><p>Positive Control: HUVEC cells</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.058 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed : 19048083).
See full target information Pecam1
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1629770 PubMed40894922

2025

Efficacy of bleomycin and sirolimus in inhibiting CD31 endothelial cell proliferation in noninvoluting congenital hemangiomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Li,Chuan Wang,Yi Li,Xinglong Zhu,Ji Bao,Yi Ji

Oncogene 44:2689-2714 PubMed40394416

2025

Erk1 is an oncoprotein that causes hepatocellular carcinoma in mice and imposes a rigorous negative feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Soudah,Alexey Baskin,Merav Darash-Yahana,Ilona Darlyuk-Saadon,Karina Smorodinsky-Atias,Tali Shalit,Wei-Ping Yu,Alon Savidor,Eli Pikarsky,David Engelberg

Photoacoustics 43:100722 PubMed40271379

2025

Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Mengtao Han,Zhiwei Xue,Mengchen Yu,Nanlin You,Yaguang Ren,Zhiqiang Xu,Zhifeng Wu,Yiming He,Zonghai Sheng,Chengbo Liu,Donghai Wang,Jingqin Chen

European journal of cell biology 63:247-54 PubMed8082649

1994

Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium.

Applications

Unspecified application

Species

Unspecified reactive species

A Vecchi,C Garlanda,M G Lampugnani,M Resnati,C Matteucci,A Stoppacciaro,H Schnurch,W Risau,L Ruco,A Mantovani
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com