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AB7388

Anti-CD31 antibody [MEC 7.46]

3

(8 Reviews)

|

(134 Publications)

Anti-CD31 antibody [MEC 7.46] (ab7388) is a rat monoclonal antibody detecting CD31 in Flow Cytometry, ICC/IF. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 130 publications
- Trusted since 2002

View Alternative Names

CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1

2 Images
Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (AB7388)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (AB7388)

Flow cytometric analysis of / fixed / permeabilized B16-F0 (Mouse melanoma epithelial cell-like, Left) / bEnd.3 (Mouse brain endothelioma, Right) cells labelling CD31 with ab7388 at 1/1000 dilution (0.1μg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control : B16-F0.Gated on viable cells.

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 7.46] (AB7388)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 7.46] (AB7388)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 cells labelling CD31 with ab7388 at 1/50 (19.32 μg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in bEnd.3 cell line. Negative control : B16-F0 (PMID : 8082649) ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MEC 7.46

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cell presents on all mouse blood vessels both in normal and inflamed or tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.

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Reactivity data

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AB204527

Mouse CD31 ELISA Kit (PECAM1)

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What is this antibody validated in?
Anti-CD31 antibody [MEC 7.46] (ab7388) is a rat recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-CD31 [MEC 7.46] (ab7388) was first used in a scientific publication in 2002 and has been cited over 130 times in peer-reviewed journals.

Reviewed by scientists
Anti-CD31 [MEC 7.46] (ab7388) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [MEC 7.46] also available for your convenience: ab7388, Carrier free - ab264486, Alexa Fluor® 488 - ab307133, Alexa Fluor® 647 - ab307262, Alexa Fluor® 594 - ab307336, PE - ab314282

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed : 19048083).
See full target information Pecam1
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Publications (134)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & medicine 20: PubMed37921408

2023

TNFSF15 facilitates the differentiation of CD11b myeloid cells into vascular pericytes in tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangxiang Gu,Yipan Zhu,Cancan Zhao,Yixin Cao,Jingying Wang,Qiangzhe Zhang,Luyuan Li

Cardiovascular diabetology 22:216 PubMed37592255

2023

Endothelial MICU1 alleviates diabetic cardiomyopathy by attenuating nitrative stress-mediated cardiac microvascular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xide Shi,Chao Liu,Jiangwei Chen,Shiqiang Zhou,Yajuan Li,Xingcheng Zhao,Jinliang Xing,Junhui Xue,Fengzhou Liu,Fei Li

Biomaterials research 27:77 PubMed37563655

2023

Exosomes derived from mir-214-3p overexpressing mesenchymal stem cells promote myocardial repair.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwu Zhu,Qingjie Wang,Jian Zhang,Ling Sun,Xiu Hong,Wei Du,Rui Duan,Jianguang Jiang,Yuan Ji,Haoran Wang,Bing Han

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23107 PubMed37534948

2023

Kinetics of skeletal muscle regeneration after mild and severe muscle damage induced by electrically-evoked lengthening contractions.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Bernard,Charline Jomard,Bénédicte Chazaud,Julien Gondin

Nature communications 14:1980 PubMed37031213

2023

Endothelial FAT1 inhibits angiogenesis by controlling YAP/TAZ protein degradation via E3 ligase MIB2.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Li,Jingchen Shao,Young-June Jin,Haruya Kawase,Yu Ting Ong,Kerstin Troidl,Qi Quan,Lei Wang,Remy Bonnavion,Astrid Wietelmann,Francoise Helmbacher,Michael Potente,Johannes Graumann,Nina Wettschureck,Stefan Offermanns

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:3439-3455 PubMed37015809

2023

Interleukin (IL)-1 Receptor Signaling Is Required for Complete Taste Bud Regeneration and the Recovery of Neural Taste Responses following Axotomy.

Applications

Unspecified application

Species

Unspecified reactive species

Guangkuo Dong,Schuyler Kogan,Natasha Venugopal,Eddy Chang,Lianying He,Fama Faal,Yang Shi,Lynnette Phillips McCluskey

Advanced biology 7:e2200320 PubMed36988414

2023

VEGFA Promotes Skeletal Muscle Regeneration in Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Yori Endo,Charles D Hwang,Yuteng Zhang,Shayan Olumi,Daniel J Koh,Christina Zhu,Ronald L Neppl,Shailesh Agarwal,Indranil Sinha

Molecular neurobiology 60:3741-3757 PubMed36940077

2023

The Aldose Reductase Inhibitor Epalrestat Maintains Blood-Brain Barrier Integrity by Enhancing Endothelial Cell Function during Cerebral Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Tongshuai Zhang,Jinrong Wu,Xinmin Yao,Yao Zhang,Yue Wang,Yang Han,Yun Wu,Zhenyu Xu,Jing Lan,Siyu Han,Haifeng Zou,Qixu Sun,Dandan Wang,Jingyu Zhang,Guangyou Wang

Journal of pharmaceutical analysis 13:262-275 PubMed37102105

2023

Overcoming chemoresistance in non-angiogenic colorectal cancer by metformin via inhibiting endothelial apoptosis and vascular immaturity.

Applications

Unspecified application

Species

Unspecified reactive species

Guang-Yue Li,Shu-Jing Zhang,Dong Xue,Yue-Qi Feng,Yan Li,Xun Huang,Qiang Cui,Bo Wang,Jun Feng,Tao Bao,Pei-Jun Liu,Shao-Ying Lu,Ji-Chang Wang

Nature genetics 55:232-245 PubMed36658434

2023

Notch1 mutations drive clonal expansion in normal esophageal epithelium but impair tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Abby,Stefan C Dentro,Michael W J Hall,Joanna C Fowler,Swee Hoe Ong,Roshan Sood,Albert Herms,Gabriel Piedrafita,Irina Abnizova,Christian W Siebel,Moritz Gerstung,Benjamin A Hall,Philip H Jones
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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