Anti-CD31 antibody [P2B1] is a mouse monoclonal antibody that is used to detect CD31 in ICC, IHC-Fr, Western blot. Suitable for Human samples.
- Trusted since 2005
Images
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [P2B1] (AB24590), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-p2b1-ab24590--immunohistochemistry-frozen-sections-img125761.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [P2B1] (AB24590)")
![Immunocytochemistry - Anti-CD31 antibody [P2B1] (AB24590), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-p2b1-ab24590--immunocytochemistry-img111517.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CD31 antibody [P2B1] (AB24590)")
Publications
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- Experimental and therapeutic medicine 26:5692023Effect of autologous lyophilized platelet‑rich fibrin on the reconstruction of osteochondral defects in rabbits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianwei Sun,Leng Han,Chundong Liu,Junli Ma,Xiao Li,Shuohui Sun,Zhifa WangPubMed 37954116
- International journal of nanomedicine 18:2923-29382023Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Dong,Bin Wu,Weidong TianPubMed 37288352
- Cell transplantation 32:96368972311704372023Combinational Treatment Involving Decellularized Extracellular Matrix Hydrogels With Mesenchymal Stem Cells Increased the Efficacy of Cell Therapy in Pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHideaki Kojima,Hiroko Kushige,Hiroshi Yagi,Takayuki Nishijima,Nobuko Moritoki,Narihito Nagoshi,Yutaka Nakano,Masayuki Tanaka,Shutaro Hori,Yasushi Hasegawa,Yuta Abe,Minoru Kitago,Masaya Nakamura,Yuko KitagawaPubMed 37191199
- iScience 26:1068302023APOL1 promotes endothelial cell activation beyond the glomerulus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiguel Carracedo,Elke Ericson,Rasmus Ågren,Anna Forslöw,Katja Madeyski-Bengtson,Anna Svensson,Rebecca Riddle,Jonas Christoffersson,Hernán González-King Garibotti,Bojana Lazovic,Ryan Hicks,Lisa Buvall,Alessia Fornoni,Peter J Greasley,Mark LalPubMed 37250770
- Journal of experimental & clinical cancer research : CR 42:852023Targeting inhibition of prognosis-related lipid metabolism genes including CYP19A1 enhances immunotherapeutic response in colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLilong Liu,Min Mo,Xuehan Chen,Dongchen Chao,Yufan Zhang,Xuewei Chen,Yang Wang,Nan Zhang,Nan He,Xi Yuan,Honglei Chen,Jing YangPubMed 37055842
- Frontiers in molecular neuroscience 16:11219442023Increased NOX2 expression in astrocytes leads to eNOS uncoupling through dihydrofolate reductase in endothelial cells after subarachnoid hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Hao Miao,Sheng-Qing Gao,Hui-Xin Li,Yun-Song Zhuang,Xue Wang,Tao Li,Chao-Chao Gao,Yan-Ling Han,Jia-Yin Qiu,Meng-Liang ZhouPubMed 37063365
- Protein & cell 14:105-1222023Resolving the lineage relationship between malignant cells and vascular cells in glioblastomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangyu Wang,Xuan Liu,Shaowen Li,Chen Zhao,Yumei Sun,Kuan Tian,Junbao Wang,Wei Li,Lichao Xu,Jing Jing,Juan Wang,Sylvia M Evans,Zhiqiang Li,Ying Liu,Yan ZhouPubMed 36929001
- Military Medical Research 10:132023Pericytes protect rats and mice from sepsis-induced injuries by maintaining vascular reactivity and barrier function: implication of miRNAs and microvesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Sen Zhang,Yi-Yan Liu,Shuang-Shuang He,Dai-Qin Bao,Hong-Chen Wang,Jie Zhang,Xiao-Yong Peng,Jia-Tao Zang,Yu Zhu,Yue Wu,Qing-Hui Li,Tao Li,Liang-Ming LiuPubMed 36907884
- International journal of bioprinting 9:7032023Integrating zinc/silicon dual ions with 3D-printed GelMA hydrogel promotes hair follicle regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanliang Zhang,Zhaowenbin Zhang,Xianlan Duan,Wei Song,Zhao Li,Bin Yao,Yi Kong,Xing Huang,Xiaobing Fu,Jiang Chang,Sha HuangPubMed 37273992
- Frontiers in pharmacology 14:10845642023Effect of Dl-3-n-butylphthalide on mitochondrial Cox7c in models of cerebral ischemia/reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Jia,Jianwen Deng,Haiqiang Jin,Jie Yang,Ding Nan,Zemou Yu,Weiwei Yu,Zhiyuan Shen,Yuxuan Lu,Ran Liu,Zhaoxia Wang,Xiaozhong Qu,Dong Qiu,Zhenzhong Yang,Yining HuangPubMed 36909178
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC | IHC-P | IHC-Fr | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Cow | Not recommended | Not recommended | Not recommended | Not recommended |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Cow | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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10 products for Alternative Product
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.
Alternative names
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Anti-CD31 antibody [P2B1] is a mouse monoclonal antibody that is used to detect CD31 in ICC, IHC-Fr, Western blot. Suitable for Human samples.
- Trusted since 2005
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- P2B1
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CD31 antibody [P2B1] (ab24590) is a mouse monoclonal antibody and is validated for use in ICC, IHC-Fr, WB in human samples.
Anti-CD31 antibody [P2B1] (ab24590) has been cited over 291 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-CD31 antibody [P2B1] (ab24590) has high sensitivity and specificity.
Anti-CD31 antibody [P2B1] (ab24590) has 32 independent reviews from customers.
Anti-CD31 antibody [P2B1] (ab24590) specifically detects CD31 (UniProt ID: P16284; Molecular weight: 80kDa) and is sold in 100 µg selling sizes.
ab24590 activates the binding of human T-lymphocytes to endothelial cells.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [P2B1] (ab24590), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-p2b1-ab24590--immunohistochemistry-frozen-sections-img125761.jpg "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [P2B1] (ab24590)")
Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [P2B1] (ab24590)
IHC image of CD31 staining in a section of frozen normal human placenta performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab24590, 0.1ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Immunocytochemistry - Anti-CD31 antibody [P2B1] (ab24590), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-p2b1-ab24590--immunocytochemistry-img111517.jpg "Immunocytochemistry - Anti-CD31 antibody [P2B1] (ab24590)")
Immunocytochemistry - Anti-CD31 antibody [P2B1] (ab24590)
ab24590 staining CD31 in HUVEC cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab24590 at 1μg/mL concentration and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a high-content analysis system (Perkin Elmer, Operetta CLS™).
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