Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free

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PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (AB56299)

Tumor vascularization and VEGF in ascites.

(A) ID8, ID8-c-MYC, or ID8-KRAS cells (2 × 10⁶) were intra-peritoneal injected into C57/BL6 mice. Mice were sacrificed when the body weight reached 23 g. Disseminations were obtained from mice when sacrificed. Tumor vascularization of each dissemination was assessed using CD31 immunohistochemistry (ab56299).

Scale bars represent 200 μm and 50 μm at low and high magnification, respectively.

Paraffin sections (4-μm-thick) of the biggest tumor sections from ID8, ID8-c-MYC, and ID8-KRAS mice were dewaxed in xylene and rehydrated through graded ethanol to water. Antigens were retrieved by boiling in 10 mM citrate buffer (pH 6.0) for 30 min. The cooled sections were incubated in a peroxidase blocking solution for 10 min to quench endogenous peroxidase. Sections were incubated in a protein blocking solution at room temperature for 10 min to block non-specific binding. Sections were then stained for Ki67 using rabbit monoclonal antibody against mouse Ki67, CD31 using a rat monoclonal antibody against mouse CD31 (ab56299, Abcam 1 : 100 dilution).

Yoshida et al PLoS One. 2016 Aug 2;11(8):e0160330. doi: 10.1371/journal.pone.0160330. eCollection 2016. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (AB56299)

ab56299 at 1/400 staining CD31 in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (AB56299)

ab56299 at 1/400 dilution, staining CD31 in LPS treated mouse heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

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PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (AB56299)

Proliferation and cell number in male wild-type (+/y) and male Mct8 knockout (−/y) placentae.

(Panel C) : Representative picture demonstrating CD31 immunoreactivity (marker of endothelial cells represented by brown staining) in the mouse placenta at E18.5. The nuclei were counterstained using hematoxylin. Endothelial cells are indicated by arrows and trophoblast cells by arrowheads.

Vasilopoulou et al PLoS One. 2013 Jun 12;8(6):e65402. doi: 10.1371/journal.pone.0065402. Print 2013. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (AB56299)

ab56299 at 1/400 staining CD31 in LPS treated mouse lung tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (AB56299)

ab56299 at 1/400 dilution, staining CD31 in LPS treated mouse liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).