Anti-CD31 antibody [RM1006] - BSA and Azide free

6 product images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746)

  This data was developed using the same antibody clone in a different buffer formulation. Anti-CD31 antibody [RM1006] ab281583
  

  IHC image of Anti-CD31 antibody [RM1006] ab281583 staining CD31 in mouse lung formalin fixed paraffin embedded tissue sections, performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The section was then incubated with Anti-CD31 antibody [RM1006] ab281583 at 1:4000 dilution (0.116 μg/ml) for 30 mins at room temperature and detected using an HRP conjugated compact polymer system (Bond™ Polymer Refine Detection). DAB was used as the chromogen. Positive staining was obtained on blood vessels in mouse lung. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).

  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746)

  This data was developed using the same antibody clone in a different buffer formulation. Anti-CD31 antibody [RM1006] ab281583.
  

  IHC image of Anti-CD31 antibody [RM1006] ab281583 staining CD31 in human placenta formalin fixed paraffin embedded tissue sections, performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The section was then incubated with Anti-CD31 antibody [RM1006] ab281583 at 1:4000 dilution (0.116 μg/ml) for 30 mins at room temperature and detected using an HRP conjugated compact polymer system (Bond™ Polymer Refine Detection). DAB was used as the chromogen. Positive staining was obtained on blood vessels in human placenta. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).

  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunoprecipitation - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746)

  This data was produced using Anti-CD31 antibody [RM1006] ab281583, the same clone in a different formulation.
  

  CD31 was immunoprecipitated from 0.35 mg THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10 ug with 281583 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 281583 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10 ug

  Lane 2: Anti-CD31 antibody [RM1006] ab281583 IP in THP-1 whole cell lysate

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD31 antibody [RM1006] ab281583 in THP-1 whole cell lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 6 seconds.

  All lanes: Immunoprecipitation - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746)

  Predicted band size: 82 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746)

  This data was developed using the same antibody clone in a different buffer formulation. Anti-CD31 antibody [RM1006] ab281583
  

  IHC image of Anti-CD31 antibody [RM1006] ab281583 staining CD31 in rat lung formalin fixed paraffin embedded tissue sections, performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The section was then incubated with Anti-CD31 antibody [RM1006] ab281583 at 1:4000 dilution (0.116 μg/ml) for 30 mins at room temperature and detected using an HRP conjugated compact polymer system (Bond™ Polymer Refine Detection). DAB was used as the chromogen. Positive staining was obtained on blood vessels in rat lung. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).

  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunoprecipitation - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746)

  This data was produced using Anti-CD31 antibody [RM1006] ab281583, the same clone in a different formulation.
  

  CD31 was immunoprecipitated from 0.35 mg bEnd.3 (Mouse brain endothelioma ) whole cell lysate 10 ug with 281583 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 281583 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: bEnd.3 (Mouse brain endothelioma ) whole cell lysate 10 ug

  Lane 2: Anti-CD31 antibody [RM1006] ab281583 IP in bEnd.3 whole cell lysate

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD31 antibody [RM1006] ab281583 in bEnd.3 whole cell lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 6 seconds.

  All lanes: Immunoprecipitation - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746)

  Predicted band size: 82 kDa

• 

  Western blot - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746)

  This data was produced using Anti-CD31 antibody [RM1006] ab281583, the same clone in a different formulation.
  

  Blocking and diluting buffer and concentration:5% NFDM/TBST

  Negative control: NIH/3T3 (PMID: 1429859).

  Exposure time: 37 seconds

  All lanes: Western blot - Anti-CD31 antibody [RM1006] - BSA and Azide free (ab282746) at 1/1000 dilution

  Lane 1: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

  Lane 2: bEnd.3 (Mouse brain endothelioma ) whole cell lysate at 20 µg

  Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

  Lane 4: Rat lung lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 82 kDa