Rabbit Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
IHC-P | Flow Cyt (Intra) | |
---|---|---|
Human | Tested | Tested |
Mouse | Predicted | Predicted |
Pig | Predicted | Predicted |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Mouse, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Pig | Dilution info - | Notes - |
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Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Rabbit Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
Purified from TCS by protein A/G.
ab243932 is the carrier-free version of Anti-CD31 antibody [SP164] - C-terminal ab227656.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) labeling CD31 with purified Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/20 dilution (5.6μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Intracellular flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD31 with Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human cervix tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human placenta tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human lung tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human skin tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD31 using Anti-CD31 antibody [SP164] - C-terminal ab227656 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD31 antibody [SP164] - C-terminal ab227656).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com