JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227656

Anti-CD31 antibody [SP164] - C-terminal

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal CD31 antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human placenta tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human cervix tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human lung tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) labeling CD31 with purified ab227656 at 1/20 dilution (5.6 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD31 with ab227656 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human skin tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP164

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p>The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.</p><p>Positive Control: Hu tonsil tissue</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/100", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information PECAM1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biochemistry and function 43:e70116 PubMed40864431

2025

The Extracellular Matrix Agrin Promotes Epicardial Cells Activation and Proliferation Post Myocardial Infarction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Han,Dinghui Wang,Huiping Yang,Xiaodong Jing,Qiang She,Bin Liu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB231436

Anti-CD31 antibody [SP38]

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP38] (AB231436)

  • 5
  • 5
Primary Antibodies

AB124432

Anti-CD31 antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB124432)

  • 5
  • 4
Primary Antibodies

AB222783

Anti-CD31 antibody [EPR17260-263]

Recombinant|RabMAb|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (AB222783)

  • 5
  • 11
Primary Antibodies

AB134168

Anti-CD31 antibody [EP3095]

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

  • 4
  • 7
Primary Antibodies

AB182981

Anti-CD31 antibody [EPR17259]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] (AB182981)

  • 5
  • 22
Primary Antibodies

AB76533

Anti-CD31 antibody [EPR3094]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (AB76533)

  • 4
  • 12
Primary Antibodies

AB271994

Anti-CD31 antibody [EPR17260-254] - BSA and Azide free

RabMAb|Recombinant|Advanced Validation

IHC-tissue clearing - Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (AB271994)

  • 0
  • 0
Primary Antibodies

AB207090

Anti-CD31 antibody [EPR3094] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (AB207090)

  • 5
  • 2
Primary Antibodies

AB226157

Anti-CD31 antibody [EP3095] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] - BSA and Azide free (AB226157)

  • 0
  • 0
Primary Antibodies

AB199734

Biotin Anti-CD31 antibody [EPR3094]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD31 antibody [EPR3094] (AB199734)

  • 0
  • 0
Primary Antibodies

AB32457

Anti-CD31 antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB32457)

  • 4
  • 12
Primary Antibodies

AB218582

Alexa Fluor® 647 Anti-CD31 antibody [EPR3094]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD31 antibody [EPR3094] (AB218582)

  • 0
  • 0

Complementary Products

Assay Kits

AB190814

Human CD31 ELISA Kit (PECAM)

Recombinant|SimpleStep

Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com