Rabbit Recombinant Monoclonal CD31 antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (AB227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--flow-cytometry-intracellular-img25810.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118404.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)")
![Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (AB227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--flow-cytometry-intracellular-img122333.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122230.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137281.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (AB227656)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
12 products for Alternative Product
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity).Isoform Delta15Does not protect against apoptosis.
Alternative names
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Rabbit Recombinant Monoclonal CD31 antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
SP164
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
13 product images
![Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--flow-cytometry-intracellular-img25810.jpg "Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) labeling CD31 with purified ab227656 at 1/20 dilution (5.6 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118404.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--flow-cytometry-intracellular-img122333.jpg "Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Flow Cytometry (Intracellular) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD31 with ab227656 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122230.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human cervix tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137281.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91922.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human placenta tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137057.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119377.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139168.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116161.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124799.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human lung tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138202.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human skin tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59111.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91922.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137057.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119377.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139168.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116161.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124799.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138202.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-sp164-c-terminal-ab227656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59111.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [SP164] - C-terminal (ab227656)")
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com