Mouse Monoclonal CD31 antibody. Suitable for IHC-Fr and reacts with Rat samples. Cited in 79 publications. Immunogen corresponding to Cell preparation containing Pecam1 protein.
Images
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-tld-3a12-ab64543--immunohistochemistry-frozen-sections-img31773.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543)")
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-tld-3a12-ab64543--immunohistochemistry-frozen-sections-img132703.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543)")
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-tld-3a12-ab64543--immunohistochemistry-frozen-sections-img102804.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543)")
Publications
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- International journal of medical sciences 21:137-1502024Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanyu Li,Hongxiao Wu,Feng Wang,Lingchi Kong,Yifan Yu,Rongtai Zuo,Haoyu Zhao,Jia Xu,Qinglin KangPubMed 38164350
- Nanoscale 15:17173-171832023Effects of graphene oxide and reduced graphene oxide nanomaterials on porcine endothelial progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Polo-Montalvo,Mónica Cicuéndez,Laura Casarrubios,Nathalie Barroca,Daniela da Silva,María José Feito,Rosalía Diez-Orejas,María Concepción Serrano,Paula A A P Marques,María Teresa PortolésPubMed 37853851
- Advanced functional materials 33:2023Simulation of Cortical and Cancellous Bone to Accelerate Tissue Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhihai Fan,Hongxiang Liu,Zhaozhao Ding,Liying Xiao,Qiang Lu,David L KaplanPubMed 37601745
- Frontiers in cardiovascular medicine 9:10130132022(Wall.) Meisn. extract protects against myocardial infarction by inhibiting NF-κB-and MAPK-mediated endothelial inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Wei,Le Tang,Lingqing Su,Sufen Zeng,Ajdora Telushi,Xiaoya Lang,Yanli Zhang,Manman Qin,Liang Qiu,Chao Zhong,Jun YuPubMed 36606274
- Neural regeneration research 18:1286-12922022Overexpression of vascular endothelial growth factor enhances the neuroprotective effects of bone marrow mesenchymal stem cell transplantation in ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCui Liu,Zhi-Xiang Yang,Si-Qi Zhou,Ding Ding,Yu-Ting Hu,Hong-Ning Yang,Dong Han,Shu-Qun Hu,Xue-Mei ZongPubMed 36453413
- Frontiers in immunology 13:9765112022Pooled human bone marrow-derived mesenchymal stromal cells with defined trophic factors cargo promote dermal wound healing in diabetic rats by improved vascularization and dynamic recruitment of M2-like macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHélène Willer,Gabriele Spohn,Kimberly Morgenroth,Corinna Thielemann,Susanne Elvers-Hornung,Peter Bugert,Bruno Delorme,Melanie Giesen,Thomas Schmitz-Rixen,Erhard Seifried,Christiane Pfarrer,Richard Schäfer,Karen BiebackPubMed 36059533
- Nanomaterials (Basel, Switzerland) 12:2022A Trilayer Dressing with Self-Pumping and pH Monitoring Properties for Promoting Abdominal Wall Defect Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Hu,Guopu Chen,Gefei WangPubMed 36014666
- Microbiology spectrum 10:e00177222022Fecal Microbiota Transplantation Exerts Neuroprotective Effects in a Mouse Spinal Cord Injury Model by Modulating the Microenvironment at the Lesion Site.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingli Jing,Fan Bai,Limiao Wang,Degang Yang,Yitong Yan,Qiuying Wang,Yanbing Zhu,Yan Yu,Zhiguo ChenPubMed 35467388
- Frontiers in behavioral neuroscience 16:8114192022Transcutaneous Auricular Vagus Nerve Stimulation Promotes White Matter Repair and Improves Dysphagia Symptoms in Cerebral Ischemia Model Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Long,Qianwen Zang,Gongwei Jia,Meng Fan,Liping Zhang,Yingqiang Qi,Yilin Liu,Lehua Yu,Sanrong WangPubMed 35493949
- Science advances 8:eabl38882022Mechanically reinforced biotubes for arterial replacement and arteriovenous grafting inspired by architectural engineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDengke Zhi,Quhan Cheng,Adam C Midgley,Qiuying Zhang,Tingting Wei,Yi Li,Ting Wang,Tengzhi Ma,Muhammad Rafique,Shuang Xia,Yuejuan Cao,Yangchun Li,Jing Li,Yongzhe Che,Meifeng Zhu,Kai Wang,Deling KongPubMed 35294246
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing Pecam1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q3SWT0
Reactivity data
Select an application| IHC-P | IHC-Fr | |
|---|---|---|
| Human | Not recommended | Not recommended |
| Rat | Not recommended | Tested |
| Pig | Not recommended | Not recommended |
| Rhesus monkey | Not recommended | Not recommended |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Pig, Rhesus monkey | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10.00000 - 1/100.00000 | Notes A fixation with acetone or methanol is recommended. Do not fix with PFA. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes A fixation with acetone or methanol is recommended. Do not fix with PFA. |
Species Pig | Dilution info - | Notes - |
Species Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.
Alternative names
CD31, Pecam, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1
Recommended products
Mouse Monoclonal CD31 antibody. Suitable for IHC-Fr and reacts with Rat samples. Cited in 79 publications. Immunogen corresponding to Cell preparation containing Pecam1 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing Pecam1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q3SWT0
- Clone number
- TLD-3A12
- Purification technique
- Affinity purification Immunogen
- Specificity
ab64543 recognises rat PECAM-1 (CD31). It also partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
This antibody has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-tld-3a12-ab64543--immunohistochemistry-frozen-sections-img31773.jpg "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)")
This image is courtesy of Valley Lasry.Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)
ab64543 staining CD31 in rat brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in paraformaldehyde prior to blocking with 5% donkey serum, BSA 1%, Triton 1.5% for 2 hours at room temperature. The primary antibody was diluted 1/200 in PBS/5% Donkey serum and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-tld-3a12-ab64543--immunohistochemistry-frozen-sections-img132703.jpg "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)")
Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)
Immunohistochemical staining of rat brain cryosection with Mouse anti Rat CD31 (ab64543)
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-tld-3a12-ab64543--immunohistochemistry-frozen-sections-img102804.jpg "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)")
Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)
Immunohistochemical staining of rat spleen cryosection with Mouse anti Rat CD31 (ab64543)
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