Anti-CD31 antibody [TLD-3A12]

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543)

Immunohistochemical staining of rat spleen cryosection with Mouse anti Rat CD31 (ab64543)

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543)

Immunohistochemical staining of rat brain cryosection with Mouse anti Rat CD31 (ab64543)

• IHC-Fr

AbReview25216****

Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (AB64543)

ab64543 staining CD31 in rat brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in paraformaldehyde prior to blocking with 5% donkey serum, BSA 1%, Triton 1.5% for 2 hours at room temperature. The primary antibody was diluted 1/200 in PBS/5% Donkey serum and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.

This image is courtesy of Valley Lasry.

• IHC

CiteAb

Immunohistochemistry - Anti-CD31 antibody [TLD-3A12] (AB64543)

Immunohistochemistry using Anti-CD31 antibody [TLD-3A12], ab64543. Publication image from Huang, S. M. et al., 2013, J Biomed Sci, 23876213. Legend direct from paper.

Analysis of angiogenesis and blood vessel density in ASC-treated tissue. (A-C) After three weeks of treatment, the tissues of the wound edge were subjected to histological examination. Compared with the control group (A), the capillary density in the dermis was clearly increased in the ASC-treated group (B). Arrows indicate vessel formation and proliferation sites containing erythrocytes in each wound. E : epidermis; M : muscle layer; U : ulcer. Bar : 500 µm. (C) Tissue lysates from re-epithelialized skin tissue of control and ASC-treated groups were analyzed with immunoblot analysis against CD31 and HuR (a loading control). (D-F) Three weeks after ASC treatment, ulcerated tissues were removed and processed into 8 µm frozen sections. Slides were stained with CD31 (green) and DAPI, a fluorescent nuclear stain (blue). Bar : 50 µm. Representative images of the control (D) and ASC (E) group are shown. (F) The blood vessel density was evaluated in the control and ASC-treated groups. The data represent the mean ± SEM of eight rats. *p < 0.01.

• IHC

CiteAb

Immunohistochemistry - Anti-CD31 antibody [TLD-3A12] (AB64543)

Immunohistochemistry using Anti-CD31 antibody [TLD-3A12], ab64543. Publication image from Huang, S. M. et al., 2013, J Biomed Sci, 23876213. Legend direct from paper.

Engrafted CM-DiI-labeled ASCs were expressed within the endothelial cells. (A-C) Images obtained from 16 µm sections frozen 14 days after the injection of CM-DiI-labeled ASCs (red fluorescence) into tissue with radiation ulcers. Phase contrast (A), red fluorescence (B) and overlay images (C). Bar : 200 µm. (D-K) Confocal images were acquired from 8 µm sections frozen 14 days after the injection of CM-DiI-labeled ASCs and immunostained with vWF (D-G) or CD31 (H-K). Differential interference contrast image (D and H). Red staining indicates CM-DiI-labeled ASCs (E and I). Green staining indicates vWF (F) and CD31 (J). Overlay image (G and K).