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AB218

Anti-CD31 antibody [WM59]

3

(1 Review)

|

(10 Publications)

Mouse Recombinant Monoclonal CD31 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples. Cited in 10 publications.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] (AB218)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] (AB218)

Immunocytochemistry analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC cells labelling CD31 with ab218 at 1/100 dilution (10.62μg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2μg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control cell : HEK-293 (PMID : 27097314).

Negative control 1 : ab218 at a 1/100 dilution (10.62μg/ml) followed by ab150080 at a 1/500 dilution (4μg/ml).

Negative control 2 : ab179513 at a 1/500 dilution (4μg/ml) followed by ab150113 at a 1/1000 dilution (4μg/ml).

Flow Cytometry - Anti-CD31 antibody [WM59] (AB218)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD31 antibody [WM59] (AB218)

Flow cytometric analysis of HEK 293 (human embryonic kidney epithelial cell) (Left panel) / HUVEC (human umbilical vein endothelial cell) (Right panel) cells labelling CD31 with ab218 at 1/1000 dilution (1.062μg/ml) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Negative control : HEK 293 (PMID : 27097314).

Gated on viable cells.

  • Carrier free

    Anti-CD31 antibody [WM59] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WM59

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10.62 µg/mL", "ICCIF-species-notes": "<p>Use 0.3M glycine to quench autofluorescence caused by aldehydes.</p><p>Positive Control: HUVEC cells</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.062 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information PECAM1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0330478 PubMed40839600

2025

MSC and HUVEC co-cultured fillers overcome intractable fistula in a new mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Soichiro Hirasawa,Kentaro Murakami,Masayuki Kano,Satoshi Endo,Takeshi Toyozumi,Yasunori Matsumoto,Ryota Otsuka,Nobufumi Sekino,Tadashi Shiraishi,Takahiro Ryuzaki,Kazuya Kinoshita,Takuma Sasaki,Hisahiro Matsubara

Oncology letters 20:303 PubMed33093912

2020

Endothelial transdifferentiation of human HGC-27 gastric cancer cells .

Applications

Unspecified application

Species

Unspecified reactive species

Changxin Chen,Zhixin Hunag,Mucheng Wang,Zicheng Huang,Xiangbo Chen,Anye Huang,Binbin Zheng,Lishan Wu,Yi Liu,Xinwen Wang,Weifeng Xu

Frontiers in immunology 9:1802 PubMed30127787

2018

T Cell Receptor-Independent, CD31/IL-17A-Driven Inflammatory Axis Shapes Synovitis in Juvenile Idiopathic Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ian D Ferguson,Patricia Griffin,Joshua J Michel,Hiroshi Yano,Sarah L Gaffen,Robert G Mueller,Jeffrey A Dvergsten,Jon D Piganelli,Margalit E Rosenkranz,Daniel A Kietz,Abbe N Vallejo

PloS one 11:e0161629 PubMed27552229

2016

The Arachidonate 15-Lipoxygenase Enzyme Product 15-HETE Is Present in Heart Tissue from Patients with Ischemic Heart Disease and Enhances Clot Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Lundqvist,Mikael Sandstedt,Joakim Sandstedt,Ruth Wickelgren,Göran I Hansson,Anders Jeppsson,Lillemor Mattsson Hultén

Biology of reproduction 90:110 PubMed24648400

2014

Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan E Bushway,Scott A Gerber,Bruce M Fenton,Richard K Miller,Edith M Lord,Shawn P Murphy

The EMBO journal 31:3431-41 PubMed22828869

2012

Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Feride Oeztuerk-Winder,Anna Guinot,Anna Ochalek,Juan-Jose Ventura

Clinical cancer research : an official journal of the American Association for Cancer Research 18:454-64 PubMed22065082

2011

Targeting tumor-associated endothelial cells: anti-VEGFR2 immunoliposomes mediate tumor vessel disruption and inhibit tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Wicki,Christoph Rochlitz,Annette Orleth,Reto Ritschard,Imke Albrecht,Richard Herrmann,Gerhard Christofori,Christoph Mamot

Molecular cancer therapeutics 7:2536-46 PubMed18723498

2008

Human endothelial precursor cells express tumor endothelial marker 1/endosialin/CD248.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca G Bagley,Cecile Rouleau,Thia St Martin,Paula Boutin,William Weber,Melanie Ruzek,Nakayuki Honma,Mariana Nacht,Srinivas Shankara,Shiro Kataoka,Isao Ishida,Bruce L Roberts,Beverly A Teicher

Blood 110:151-60 PubMed17379743

2007

Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.

Applications

Unspecified application

Species

Unspecified reactive species

Masumi Nagano,Toshiharu Yamashita,Hiromi Hamada,Kinuko Ohneda,Ken-ichi Kimura,Tomoki Nakagawa,Masabumi Shibuya,Hiroyuki Yoshikawa,Osamu Ohneda

FEBS letters 579:2569-75 PubMed15862292

2005

Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium.

Applications

Unspecified application

Species

Unspecified reactive species

John R MacFadyen,Oliver Haworth,David Roberston,Deborah Hardie,Marie-Therese Webster,Howard R Morris,Maria Panico,Mark Sutton-Smith,Anne Dell,Peter van der Geer,Dirk Wienke,Christopher D Buckley,Clare M Isacke
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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