Mouse Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples. Cited in 6 publications.
Images
![Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (AB252265), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-wm59-bsa-and-azide-free-ab252265--flow-cytometry-img62967.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (AB252265)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (AB252265), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-wm59-bsa-and-azide-free-ab252265--immunocytochemistry-immunofluorescence-img86783.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (AB252265)")
Publications
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- PloS one 11:e01616292016The Arachidonate 15-Lipoxygenase Enzyme Product 15-HETE Is Present in Heart Tissue from Patients with Ischemic Heart Disease and Enhances Clot Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnika Lundqvist et. al.PubMed 27552229
- Biology of reproduction 90:1102014Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeghan E Bushway et. al.PubMed 24648400
- The EMBO journal 31:3431-412012Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeride Oeztuerk-Winder et. al.PubMed 22828869
- Clinical cancer research : an official journal of the American Association for Cancer Research 18:454-642011Targeting tumor-associated endothelial cells: anti-VEGFR2 immunoliposomes mediate tumor vessel disruption and inhibit tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndreas Wicki,Christoph Rochlitz,Annette Orleth,Reto Ritschard,Imke Albrecht,Richard Herrmann,Gerhard Christofori,Christoph MamotPubMed 22065082
- Blood 110:151-602007Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasumi Nagano et. al.PubMed 17379743
- FEBS letters 579:2569-752005Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn R MacFadyen et. al.PubMed 15862292
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.
Alternative names
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Mouse Recombinant Monoclonal CD31 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- WM59
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab252265 is the carrier-free version of Anti-CD31 antibody [WM59] ab218.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-wm59-bsa-and-azide-free-ab252265--flow-cytometry-img62967.jpg "Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)")
Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
Flow cytometric analysis of HEK 293 (human embryonic kidney epithelial cell) (Left panel) / HUVEC (human umbilical vein endothelial cell) (Right panel) cells labelling CD31 with Anti-CD31 antibody [WM59] ab218 at 1/1000 dilution (1.062μg/ml) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control: HEK 293 (PMID: 27097314).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [WM59] ab218).
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265), expandable thumbnail](https://content.abcam.com/products/images/cd31-antibody-wm59-bsa-and-azide-free-ab252265--immunocytochemistry-immunofluorescence-img86783.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)")
Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC cells labelling CD31 with Anti-CD31 antibody [WM59] ab218 at 1/100 dilution (10.62μg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2μg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control cell: HEK-293 (PMID: 27097314).
Negative control 1: Anti-CD31 antibody [WM59] ab218 at a 1/100 dilution (10.62μg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/500 dilution (4μg/ml).
Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 at a 1/500 dilution (4μg/ml) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at a 1/1000 dilution (4μg/ml).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD31 antibody [WM59] ab218).
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