Anti-CD31 antibody [WM59] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (AB252265)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC cells labelling CD31 with ab218 at 1/100 dilution (10.62μg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 (2μg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control cell : HEK-293 (PMID : 27097314).

Negative control 1 : ab218 at a 1/100 dilution (10.62μg/ml) followed by ab150080 at a 1/500 dilution (4μg/ml).

Negative control 2 : ab179513 at a 1/500 dilution (4μg/ml) followed by ab150113 at a 1/1000 dilution (4μg/ml).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218).

• Flow Cyt

Unknown

Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (AB252265)

Flow cytometric analysis of HEK 293 (human embryonic kidney epithelial cell) (Left panel) / HUVEC (human umbilical vein endothelial cell) (Right panel) cells labelling CD31 with ab218 at 1/1000 dilution (1.062μg/ml) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Negative control : HEK 293 (PMID : 27097314).

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218).