Rabbit Polyclonal CD31 phospho Y713 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CD31 phospho Y713 aa 650 to C-terminus.
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Publications
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- Aging cell 20:e135012021Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengrong Zhang,Hana Na,Qini Gan,Qiushan Tao,Yuriy Alekseyev,Junming Hu,Zili Yan,Jack B Yang,Hua Tian,Shenyu Zhu,Qiang Li,Ibraheem M Rajab,Jan Krizysztof Blusztajn,Benjamin Wolozin,Andrew Emili,Xiaoling Zhang,Thor Stein,Lawrence A Potempa,Wei Qiao QiuPubMed 34687487
- Journal of molecular and cellular cardiology 49:771-802010Human progenitor cells derived from cardiac adipose tissue ameliorate myocardial infarction in rodents.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human CD31 phospho Y713 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P16284
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Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Hu Isoform 1-6: 79-83 kDa (predicted) Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
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Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity).Isoform Delta15Does not protect against apoptosis.
Alternative names
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Rabbit Polyclonal CD31 phospho Y713 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CD31 phospho Y713 aa 650 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human CD31 phospho Y713 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P16284
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CD31 (phospho Y713) antibody (ab62169)
All lanes: Western blot - Anti-CD31 (phospho Y713) antibody (ab62169) at 1/500 dilution
All lanes: HepG2 cells
Predicted band size: 82 kDa
Immunocytochemistry - Anti-CD31 (phospho Y713) antibody (ab62169)
Immunocytochemistry analysis of HeLa cells, using CD31 (phospho Y713) antibody (ab62169) at 1/100 - 1/500 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.
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