AB62169

Anti-CD31 (phospho Y713) antibody

Lab Essentials
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(3 Publications)

Rabbit Polyclonal CD31 phospho Y713 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CD31 phospho Y713 aa 650 to C-terminus.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

2 Images

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Immunocytochemistry - Anti-CD31 (phospho Y713) antibody (AB62169)

Immunocytochemistry analysis of HeLa cells, using CD31 (phospho Y713) antibody (ab62169) at 1/100 - 1/500 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

Supplier Data

Western blot - Anti-CD31 (phospho Y713) antibody (AB62169)

All lanes:

Western blot - Anti-CD31 (phospho Y713) antibody (ab62169) at 1/500 dilution

Lane 1:

HepG2 cells

Lane 2:

HepG2 cells with blocked with the phospho peptide

Predicted band size: 82 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human CD31 phospho Y713 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P16284

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "Use 0.3M glycine to quench autofluorescence caused by aldehydes.Positive Control: HUVEC cells", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.Hu Isoform 1-6: 79-83 kDa (predicted)Ms Isoform 1-4: 69.8-81.3 kDa (predicted)Observed band size is around 130 kDaPositive Control: HUVEC and Jurkat cell lysates (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>); Human spleen and kidney tissue lysate.Negative Control: NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>)." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.

See full target information CD31 phospho Y713

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Angiogenesis 27:861-881 PubMed39276310

2024

The influences of ApoE isoforms on endothelial adherens junctions and actin cytoskeleton responding to mCRP.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengrong Zhang,Weiwei Lin,Qini Gan,Maohua Lei,Bin Gong,Chao Zhang,Jessica Salles Henrique,Jingyan Han,Hua Tian,Qiushan Tao,Lawrence A Potempa,Thor D Stein,Andrew Emili,Wei Qiao Qiu

Aging cell 20:e13501 PubMed34687487

2021

Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Applications

Unspecified application

Species

Unspecified reactive species

Zhengrong Zhang,Hana Na,Qini Gan,Qiushan Tao,Yuriy Alekseyev,Junming Hu,Zili Yan,Jack B Yang,Hua Tian,Shenyu Zhu,Qiang Li,Ibraheem M Rajab,Jan Krizysztof Blusztajn,Benjamin Wolozin,Andrew Emili,Xiaoling Zhang,Thor Stein,Lawrence A Potempa,Wei Qiao Qiu

Journal of molecular and cellular cardiology 49:771-80 PubMed20713059

2010

Human progenitor cells derived from cardiac adipose tissue ameliorate myocardial infarction in rodents.

Applications

ICC

Species

Human

Antoni Bayes-Genis,Carolina Soler-Botija,Jordi Farré,Pilar Sepúlveda,Angel Raya,Santiago Roura,Cristina Prat-Vidal,Carolina Gálvez-Montón,José Anastasio Montero,Dirk Büscher,Juan Carlos Izpisúa Belmonte

View all publications

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Product promise

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