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AB180175

Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal

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(3 Publications )

Rabbit Recombinant Monoclonal CD31 phospho Y713 antibody. C-terminal. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 3 publications.
2 Images
Western blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (AB180175)
  • WB

Supplier Data

Western blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (AB180175)

All lanes:

Western blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175) at 1/1000 dilution

Lane 1:

Non-treated Jurkat cell lysate at 10 µg

Lane 2:

Pervanadate-treated Jurkat cell lysate at 10 µg

Secondary

All lanes:

Standard HRP goat anti-rabbit at 1/2000 dilution

Predicted band size: 82 kDa

true

Dot Blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (AB180175)
  • Dot

Lab

Dot Blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (AB180175)

Dot blot analysis of CD31 (pY713) peptide (Lane 1) and CD31 non-phospho peptide (Lane 2) labelling CD31 (phospho Y713) with ab180175 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

  • Carrier free

    Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8079(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.</p><p>The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.</p><p>Hu Isoform 1-6: 79-83 kDa (predicted).</p><p>Observed band size is around 130 kDa.</p><p>Positive Control: HUVEC and Jurkat cell lysates (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>); Human spleen and kidney tissue lysate.</p><p>Negative Control: NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>).</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information PECAM1 pY713

Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

Publications (3)

Recent publications for all applications. Explore the full listand refine your search

eLife 12: PubMed37549051

2023

CD31 signaling promotes the detachment at the uropod of extravasating neutrophils allowing their migration to sites of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Andreata,Marc Clément,Robert A Benson,Juliette Hadchouel,Emanuele Procopio,Guillaume Even,Julie Vorbe,Samira Benadda,Véronique Ollivier,Benoit Ho-Tin-Noe,Marie Le Borgne,Pasquale Maffia,Antonino Nicoletti,Giuseppina Caligiuri

Cancer cell international 20:1 PubMed31908598

2020

Patient-derived xenografts of different grade gliomas retain the heterogeneous histological and genetic features of human gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxin Zeng,Zhaohua Tang,Yongguo Li,Guangnian Yin,Zili Liu,Jie Gao,Yan Chen,Feilan Chen

Oncology letters 9:1089-1094 PubMed25663861

2015

Cross-talk between endothelial and tumor cells via basic fibroblast growth factor and vascular endothelial growth factor signaling promotes lung cancer growth and angiogenesis.

Applications

IF

Species

Unspecified reactive species

Heng DU,Hui Shi,Dali Chen,Yubin Zhou,Guowei Che
View all publications

Product promise

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