Mouse Recombinant Monoclonal FCGR2 antibody. Suitable for Flow Cyt, ICC/IF, IHC-Fr and reacts with Mouse samples. Cited in 2 publications.
Images
![Flow Cytometry - Anti-CD32 antibody [AT130-2] (AB282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--flow-cytometry-img167185.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD32 antibody [AT130-2] (AB282740)")
![Immunohistochemistry (Frozen sections) - Anti-CD32 antibody [AT130-2] (AB282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--immunohistochemistry-frozen-sections-img169851.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD32 antibody [AT130-2] (AB282740)")
![Flow Cytometry - Anti-CD32 antibody [AT130-2] (AB282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--flow-cytometry-img166351.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD32 antibody [AT130-2] (AB282740)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD32 antibody [AT130-2] (AB282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--immunocytochemistry-immunofluorescence-img171697.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD32 antibody [AT130-2] (AB282740)")
![Flow Cytometry - Anti-CD32 antibody [AT130-2] (AB282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--flow-cytometry-img173766.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD32 antibody [AT130-2] (AB282740)")
Publications
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- International journal of molecular sciences 23:2022Nicotinamide n-Oxide Attenuates HSV-1-Induced Microglial Inflammation through Sirtuin-1/NF-κB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowei Song,Wenyan Cao,Zexu Wang,Feng Li,Ji Xiao,Qiongzhen Zeng,Yuan Wang,Shan Li,Cuifang Ye,Yifei Wang,Kai ZhengPubMed 36555725
- European journal of immunology 42:2109-202012Development and characterisation of monoclonal antibodies specific for the murine inhibitory FcγRIIB (CD32B).Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily L Williams,Alison L Tutt,Ruth R French,H T Claude Chan,Betty Lau,Christine A Penfold,C Ian Mockridge,Ali Roghanian,Kerry L Cox,J Sjef Verbeek,Martin J Glennie,Mark S CraggPubMed 22760702
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Target data
Function
Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
Alternative names
CD32, Fcgr2b, Ly-17, Fcgr2, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, IgG Fc receptor II beta, Lymphocyte antigen 17, Fc-gamma RII, Fc-gamma-RIIB, FcRII
Recommended products
Mouse Recombinant Monoclonal FCGR2 antibody. Suitable for Flow Cyt, ICC/IF, IHC-Fr and reacts with Mouse samples. Cited in 2 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- AT130-2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--flow-cytometry-img167185.jpg "Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740)")
Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740)
Flow cytometric analysis of bEnd.3 (mouse brain endothelioma, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte macrophage, Right) labelling CD32 with ab282740 at 1/1000 dilution (0.1 μg), followed by Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) antibody at 1/2000 dilution (Red) compared with Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells.
Negative control: bEnd.3.
![Immunohistochemistry (Frozen sections) - Anti-CD32 antibody [AT130-2] (ab282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--immunohistochemistry-frozen-sections-img169851.jpg "Immunohistochemistry (Frozen sections) - Anti-CD32 antibody [AT130-2] (ab282740)")
Immunohistochemistry (Frozen sections) - Anti-CD32 antibody [AT130-2] (ab282740)
Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilised Mouse spleen (fresh) tissue labeling CD32 with ab282740 at 1/100 (5.0 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody. Positive staining on mouse spleen. Counterstained with DAPI.
![Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--flow-cytometry-img166351.jpg "Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740)")
Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740)
Flow cytometric analysis of mouse splenocytes cells labelling CD32 with ab282740 at 1/1000 dilution (0.1 μg / Right) compared with a mouse monoclonal IgG (Left) isotype control. Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) was used as a secondary antibody at 1/2000 dilution.
Cells were stained with mouse IgG or ab282740. Then stained with rabbit anti-mouse CD19 (Anti-CD19 antibody [EPR23174-145] - BSA and Azide free ab267394) conjugated to APC. The conjugation was performed by using APC Conjugation Kit - Lightning-Link® (APC Conjugation Kit - Lightning-Link® ab201807).
Gated on viable cells.![Immunocytochemistry/ Immunofluorescence - Anti-CD32 antibody [AT130-2] (ab282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--immunocytochemistry-immunofluorescence-img171697.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD32 antibody [AT130-2] (ab282740)")
Immunocytochemistry/ Immunofluorescence - Anti-CD32 antibody [AT130-2] (ab282740)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse spleen cells labelling CD32 with ab282740 at 1/50 dilution (18.92 μg/ml) (Green). Confocal image showing cytoplasmic staining in mouse spleen cells. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was used as a secondary at 1/1000 dilution (2 μg/ml). Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) secondary counterstain at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Negative control 1: secondary counterstain antibody only control.
Negative control 2: primary counterstain and target secondary antibody control.
![Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740), expandable thumbnail](https://content.abcam.com/products/images/cd32-antibody-at130-2-ab282740--flow-cytometry-img173766.jpg "Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740)")
Flow Cytometry - Anti-CD32 antibody [AT130-2] (ab282740)
Flow cytometric analysis of mouse splenocytes cells labelling CD32 with ab282740 at 1/1000 dilution (0.1 μg / Right) compared with a Mouse monoclonal IgG (Left) isotype control. Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) was used as a secondary antibody at 1/2000 dilution.
Gated on viable cells.
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