AB281897

Anti-CD32 antibody [AT130-5] - Rabbit IgG (Chimeric)

Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal FCGR2 antibody. Suitable for ICC and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Mouse Fcgr2.

View Alternative Names

CD32, Fcgr2b, Ly-17, Fcgr2, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, IgG Fc receptor II beta, Lymphocyte antigen 17, Fc-gamma RII, Fc-gamma-RIIB, FcRII

1 Images

Supplier Data

Immunocytochemistry - Anti-CD32 antibody [AT130-5] - Rabbit IgG (Chimeric) (AB281897)

Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD32 with ab281897 at 10 μg/ml for 1h followed by Alexa Fluor^® 488 secondary antibody at 1 μg/ml. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab281897, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor^®488 secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

AT130-5

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

ICC

applications

Immunogen

Recombinant Protein within Mouse Fcgr2. The exact immunogen used to generate this antibody is proprietary information.

P08101

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>" } } }

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Product details

ab281897 is the chimeric rabbit version of ab281896.

This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.

See full target information Fcgr2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1611637 PubMed40693271

2025

Antagonizing IL-6 receptor restores pancreatic tissue resident NK cells activation and ameliorates pancreatic injury in the mouse model of MASH.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Diana Abu Arra,Ahmad Salhab,Faris Kayed,Muneer Maali,Raghad Shweiki,Mustafa Ghanim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD32 antibody [AT130-5] - Rabbit IgG (Chimeric)

Immunocytochemistry - Anti-CD32 antibody [AT130-5] - Rabbit IgG (Chimeric) (AB281897)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise