AB125013

Anti-CD32A + CD32C antibody [EPR6658]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal FCG2A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD32, FCG2, FCGR2A1, IGFR2, FCGR2A, Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

ab125013 at 1/250 staining CD32A + CD32C in paraffin-embedded Human spleen tissue by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

ab125013 at 1/250 staining CD32A + CD32C in paraffin-embedded Human lung tissue by immunohistochemistry. Heat mediated antigen retrieval was perrformed before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

All lanes:

Western blot - Anti-CD32A + CD32C antibody [EPR6658] (ab125013) at 1/1000 dilution

Lane 1:

K562 lysate at 10 µg

Lane 2:

Human platelet lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Observed band size: 37-43 kDa

false

OI-RD Scanning - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-CD32A + CD32C antibody [EPR6658] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6658

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.

See full target information FCGR2A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BioFactors (Oxford, England) 47:865-878 PubMed34339079

2021

CDCA7-regulated inflammatory mechanism through TLR4/NF-κB signaling pathway in stomach adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Guo,Kaimei Zhou,Xiang Zhuang,Junjie Li,Xianglin Shen

Aging cell 17:e12825 PubMed30094915

2018

A molecular signature for delayed graft function.

Applications

Unspecified application

Species

Unspecified reactive species

Dagmara McGuinness,Suhaib Mohammed,Laura Monaghan,Paul A Wilson,David B Kingsmore,Oliver Shapter,Karen S Stevenson,Shana M Coley,Luke Devey,Robert B Kirkpatrick,Paul G Shiels

Investigative ophthalmology & visual science 55:247-58 PubMed24334446

2014

Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.

Applications

IHC-P

Species

Human

Salome Murinello,Robert F Mullins,Andrew J Lotery,V Hugh Perry,Jessica L Teeling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD32A + CD32C antibody [EPR6658]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

Western blot - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

OI-RD Scanning - Anti-CD32A + CD32C antibody [EPR6658] (AB125013)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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