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AB290737

Anti-CD32B antibody [AT130-5]

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(1 Publication)

Mouse Recombinant Monoclonal FCGR2 antibody. Suitable for Dot, ICC, IHC-Fr, Flow Cyt and reacts with Mouse, Recombinant full length protein - Mouse samples. Cited in 1 publication.
4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD32B antibody [AT130-5] (AB290737)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD32B antibody [AT130-5] (AB290737)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes cells labelling CD32B with ab290737 at 1/250 dilution (4.44 μg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing membranous and cytoplasmic staining in subsets of mouse splenocytes is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200  dilution (10 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue). The negative controls are as follows : -ve control 1 : ab290737 at 1/250 dilution followed by ab150080 at 1/500 dilution. -ve control 2 : ab179513 at 1/200 dilution followed by 150117 at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-CD32B antibody [AT130-5] (AB290737)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD32B antibody [AT130-5] (AB290737)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen (fresh) tissue labeling CD32B with ab290737 at 1/100 (11.1 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μg/ml) (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : PBS was used instead of primary antibody followed by secondary antibody ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)at 1/1000 dilution (2 μg/ml).

Flow Cytometry - Anti-CD32B antibody [AT130-5] (AB290737)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD32B antibody [AT130-5] (AB290737)

Flow cytometric analysis of mouse peripheral blood mononuclear cells (PBMCs) cells labelling CD32B with ab290737 at 1/1000 dilution (0.1 μg)/ Right compared with a mouse monoclonal IgG / Left isotype control.  Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/5000 dilution was used as the secondary antibody. Cells were stained with mouse IgG or ab290737, then stained with rabbit anti-mouse CD19 (ab267394) conjugated to APC. The conjugation was performed by using APC Conjugation Kit - Lightning-Link® (ab201807). Gated on viable cells.

Dot Blot - Anti-CD32B antibody [AT130-5] (AB290737)
  • Dot

Supplier Data

Dot Blot - Anti-CD32B antibody [AT130-5] (AB290737)

Dot blot analysis of CD32B using ab290737 at 1 : 1000 (0.538 μg/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 10,000 dilution.  Lane 1 : His-tag mouse Recombinant CD16/FCGR3 Protein Lane 2 : His-tag mouse Recombinant CD32B/FCGR2B Protein Exposure time : 3 minutes Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Dot Blot - Anti-CD32B antibody [AT130-5] (ab290737) at 1/1000 dilution

Lane 1:

His-tag mouse Recombinant CD16/FCGR3 Protein

Lane 2:

His-tag mouse Recombinant CD32B/FCGR2B Protein

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 3min

  • Carrier free

    Anti-CD32B antibody [AT130-5] (BSA and Azide free)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AT130-5

Isotype

IgG1

Carrier free

No

Reacts with

Mouse

Applications

ICC, IHC-Fr, Dot, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "1/250", "ICC-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Recombinant full length protein - Mouse": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
See full target information Fcgr2

Alternative Names

CD32, Fcgr2b, Ly-17, Fcgr2, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, IgG Fc receptor II beta, Lymphocyte antigen 17, Fc-gamma RII, Fc-gamma-RIIB, FcRII
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

European journal of immunology 42:2109-20 PubMed22760702

2012

Development and characterisation of monoclonal antibodies specific for the murine inhibitory FcγRIIB (CD32B).

Applications

Unspecified application

Species

Unspecified reactive species

Emily L Williams,Alison L Tutt,Ruth R French,H T Claude Chan,Betty Lau,Christine A Penfold,C Ian Mockridge,Ali Roghanian,Kerry L Cox,J Sjef Verbeek,Martin J Glennie,Mark S Cragg
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com