Rabbit Monoclonal FCG2B antibody. Suitable for IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples. Cited in 19 publications.
Images
![Western blot - Anti-CD32B antibody [EP888Y] (AB45143), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-ab45143--western-blot-img146325.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD32B antibody [EP888Y] (AB45143)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (AB45143), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-ab45143--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90996.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (AB45143)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (AB45143), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-ab45143--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100152.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (AB45143)")
![Western blot - Anti-CD32B antibody [EP888Y] (AB45143), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-ab45143--western-blot-img28751.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD32B antibody [EP888Y] (AB45143)")
Publications
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- Frontiers in pharmacology 13:8838352022Dihydroarteannuin Ameliorates Collagen-Induced Arthritis Inhibiting B Cell Activation by Activating the FcγRIIb/Lyn/SHP-1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCongqi Hu,Danbin Wu,Jiahui Yu,Jia Xu,Lijuan Liu,Mingying Zhang,Wei Jiao,Guangxing ChenPubMed 35592412
- Cancer immunology research 7:1876-18902019Cyclophosphamide Enhances Cancer Antibody Immunotherapy in the Resistant Bone Marrow Niche by Modulating Macrophage FcγR Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAli Roghanian,Guangan Hu,Christopher Fraser,Maneesh Singh,Russell B Foxall,Matthew J Meyer,Emma Lees,Heather Huet,Martin J Glennie,Stephen A Beers,Sean H Lim,Margaret Ashton-Key,Stephen M Thirdborough,Mark S Cragg,Jianzhu ChenPubMed 31451483
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:4300-43132018Amelioration of amyloid β-FcγRIIb neurotoxicity and tau pathologies by targeting LYN.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoungdae Gwon,Seo-Hyun Kim,Hyun Tae Kim,Tae-In Kam,Jisu Park,Bitna Lim,Hyunju Cha,Ho-Jin Chang,Yong Rae Hong,Yong-Keun JungPubMed 30540497
- Nature communications 9:40012018Genome-wide discovery of somatic regulatory variants in diffuse large B-cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah E Arthur et. al.PubMed 30275490
- The Journal of infectious diseases 218:1272-12832018Vaccine-Associated Maintenance of Epithelial Integrity Correlated With Protection Against Virus Entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL Shang,A J Smith,L Duan,K E Perkey,S Wietgrefe,M Zupancic,P J Southern,R P Johnson,J V Carlis,A T HaasePubMed 29401315
- Cancer research 77:5808-58192017Exosomes from Glioma-Associated Mesenchymal Stem Cells Increase the Tumorigenicity of Glioma Stem-like Cells via Transfer of miR-1587.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJavier Figueroa et. al.PubMed 28855213
- Mucosal immunology 11:512-5222017Vaccine-modified NF-kB and GR signaling in cervicovaginal epithelium correlates with protection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL Shang et. al.PubMed 28792003
- Journal of immunology (Baltimore, Md. : 1950) 193:3126-332014Live simian immunodeficiency virus vaccine correlate of protection: immune complex-inhibitory Fc receptor interactions that reduce target cell availability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnthony J Smith et. al.PubMed 25143442
- Investigative ophthalmology & visual science 55:247-582014Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.
- Modern pathology : an official journal of the Unit 27:631-92013Stromal signatures in endometrioid endometrial carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIñigo Espinosa et. al.PubMed 24263966
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Transfected cell lysate - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Transfected cell lysate - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species Transfected cell lysate - Human | Dilution info 1/10000 - 1/50000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
Alternative names
CD32, FCG2, IGFR2, FCGR2B, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b
Recommended products
Rabbit Monoclonal FCG2B antibody. Suitable for IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples. Cited in 19 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP888Y
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody has weak cross-reactivity with CD32A and CD32C. It detects weak signal in CD32A and CD32C over-expressed lysates at the dilution of 1/200 under the exposure time of 3 minutes, but detects strong band in CD32B over-expressed lysates at the dilution of 1:2000 under the exposure time of 15 seconds.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CD32B antibody [EP888Y] (ab45143), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-ab45143--western-blot-img146325.jpg "Western blot - Anti-CD32B antibody [EP888Y] (ab45143)")
Western blot - Anti-CD32B antibody [EP888Y] (ab45143)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-CD32B antibody [EP888Y] (ab45143) at 1/10000 dilution
Lane 1: Human thyroid lysate at 20 µg
Lane 2: Daudi whole cell lysate at 20 µg
SecondaryAll lanes: Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 40-44 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-ab45143--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90996.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143)
Immunohistochemical staining of paraffin embedded human spleen with purified ab45143 at a working dilution of 1/1000. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-ab45143--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100152.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143)
Unpurified ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-CD32B antibody [EP888Y] (ab45143), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-ab45143--western-blot-img28751.jpg "Western blot - Anti-CD32B antibody [EP888Y] (ab45143)")
Western blot - Anti-CD32B antibody [EP888Y] (ab45143)
Exposure times: Lanes 1 and 2: 3 minutes, Lanes 3 and 4: 15 seconds, Lanes 5 and 6: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
Lanes 1, 2, 5 and 6: Western blot - Anti-CD32B antibody [EP888Y] (ab45143) at 1/200 dilution
Lanes 3 - 4: Western blot - Anti-CD32B antibody [EP888Y] (ab45143) at 1/2000 dilution
Lane 1: Human CD32A overexpressed lysate at 10 µg
Lanes 2, 4 and 6: Non-transfected lysate at 10 µg
Lane 3: Human CD32B overexpressed lysate at 10 µg
Lane 5: Human CD32C overexpressed lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 39 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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