Rabbit Monoclonal FCG2B antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (AB206217), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-bsa-and-azide-free-ab206217--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90996.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (AB206217)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (AB206217), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-bsa-and-azide-free-ab206217--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100152.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (AB206217)")
![Western blot - Anti-CD32B antibody [EP888Y] - BSA and Azide free (AB206217), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-bsa-and-azide-free-ab206217--western-blot-img53280.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD32B antibody [EP888Y] - BSA and Azide free (AB206217)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Please check the parent abID, Anti-CD32B antibody [EP888Y] ab45143, for more information on dilutions. |
Associated Products
Select an associated product type
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1 products for Alternative Version
Target data
Function
Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
Alternative names
Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b, IGFR2, FCG2, CD32, FCGR2B
Recommended products
Rabbit Monoclonal FCG2B antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Alternative names
Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b, IGFR2, FCG2, CD32, FCGR2B
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP888Y
- Purification technique
Affinity purification Protein A
- Specificity
This antibody has weak cross-reactivity with CD32A and CD32C. It detects weak signal in CD32A and CD32C over-expressed lysates at the dilution of 1/200 under the exposure time of 3 minutes, but detects strong band in CD32B over-expressed lysates at the dilution of 1:2000 under the exposure time of 15 seconds.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab206217 is the carrier-free version of Anti-CD32B antibody [EP888Y] ab45143.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-bsa-and-azide-free-ab206217--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90996.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217)
Immunohistochemical staining of paraffin embedded human spleen with purified Anti-CD32B antibody [EP888Y] ab45143 at a working dilution of 1/1000. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD32B antibody [EP888Y] ab45143).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-bsa-and-azide-free-ab206217--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100152.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217)
Unpurified Anti-CD32B antibody [EP888Y] ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD32B antibody [EP888Y] ab45143).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217), expandable thumbnail](https://content.abcam.com/products/images/cd32b-antibody-ep888y-bsa-and-azide-free-ab206217--western-blot-img53280.jpg "Western blot - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217)")
Western blot - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-CD32B antibody [EP888Y] - BSA and Azide free (ab206217)
All lanes: Daudi (human Burkitt's lymphoma) whole cell lysates at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Predicted band size: 34 kDa
Exposure time: 8s
Downloads
Product protocols
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