Rabbit Recombinant Monoclonal FCG2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-CD32B + CD32A + CD32C antibody [EP6243] (AB131051), expandable thumbnail](https://content.abcam.com/products/images/cd32b-cd32a-cd32c-antibody-ep6243-ab131051--western-blot-img421704.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD32B + CD32A + CD32C antibody [EP6243] (AB131051)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (AB131051), expandable thumbnail](https://content.abcam.com/products/images/cd32b-cd32a-cd32c-antibody-ep6243-ab131051--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421705.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (AB131051)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (AB131051), expandable thumbnail](https://content.abcam.com/products/images/cd32b-cd32a-cd32c-antibody-ep6243-ab131051--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421706.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (AB131051)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD32B + CD32A + CD32C antibody [EP6243] (AB131051), expandable thumbnail](https://content.abcam.com/products/images/cd32b-cd32a-cd32c-antibody-ep6243-ab131051--immunocytochemistry-immunofluorescence-img421707.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD32B + CD32A + CD32C antibody [EP6243] (AB131051)")
Publications
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- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22033152022Scar-Degrading Endothelial Cells as a Treatment for Advanced Liver Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Zhao,Tian Sun,Cheng Lyu,Kaini Liang,Yudi Niu,Yuying Zhang,Chenhui Cao,Canhong Xiang,Yanan DuPubMed 36494102
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
Target data
Function
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Additional Targets
FCGR2B, FCGR2C
Alternative names
CD32, FCG2, FCGR2A1, IGFR2, FCGR2A, Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a
Recommended products
Rabbit Recombinant Monoclonal FCG2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP6243
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 4.4 x 10-12 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
This product has switched from a hybridoma to recombinant production method on 13th September 2023.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051), expandable thumbnail](https://content.abcam.com/products/images/cd32b-cd32a-cd32c-antibody-ep6243-ab131051--western-blot-img421704.jpg "Western blot - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)")
Western blot - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 40s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051), expandable thumbnail](https://content.abcam.com/products/images/cd32b-cd32a-cd32c-antibody-ep6243-ab131051--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421705.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD32B + CD32A + CD32C with ab131051 at 1/2000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051), expandable thumbnail](https://content.abcam.com/products/images/cd32b-cd32a-cd32c-antibody-ep6243-ab131051--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421706.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)
Immunohistochemical analysis of paraffin-embedded Human liver tonsil labeling CD32B + CD32A + CD32C with ab131051 at 1/2000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051), expandable thumbnail](https://content.abcam.com/products/images/cd32b-cd32a-cd32c-antibody-ep6243-ab131051--immunocytochemistry-immunofluorescence-img421707.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)")
Immunocytochemistry/ Immunofluorescence - Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji cells labelling CD32B + CD32A + CD32C with ab131051 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution, was co-incubated with ab131051 overnight at 4° C. Nucleus were visualized using DAPI.
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