AB74092

Anti-CD32B (phospho Y292) antibody

Name: Anti-CD32B (phospho Y292) antibody (ab74092) | Abcam
Brand: Abcam Limited
SKU: AB74092

(1 Review)

(1 Publication)

Rabbit Polyclonal FCG2B phospho Y292 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FCGR2B pY292.

View Alternative Names

CD32, FCG2, IGFR2, FCGR2B, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b

1 Images

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Western blot - Anti-CD32B (phospho Y292) antibody (AB74092)

All lanes:

Western blot - Anti-CD32B (phospho Y292) antibody (ab74092) at 1/500 dilution

Lane 1:

K562 cell extracts treated with PMA (125ng/ml, 30mins) at 30 µg

Lane 2:

K562 cell extracts treated with PMA (125ng/ml, 30mins) at 30 µg with immunising peptide

Predicted band size: 34 kDa

Observed band size: 34 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human FCGR2B pY292. The exact immunogen used to generate this antibody is proprietary information.

P31994

Specificity

ab74092 detects endogenous levels of CD32 only when phosphorylated at tyrosine 292.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab74092 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

See full target information FCGR2B pY292

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 29:3151-3161 PubMed37363966

2023

Dual Targeting of Apoptotic and Signaling Pathways in T-Lineage Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Caner Saygin,Giorgia Giordano,Kathryn Shimamoto,Bart Eisfelder,Anika Thomas-Toth,Girish Venkataraman,Vijayalakshmi Ananthanarayanan,Tiffaney L Vincent,Adam DuVall,Anand A Patel,Yi Chen,Fenlai Tan,Stephen P Anthony,Yu Chen,Yue Shen,Olatoyosi Odenike,David T Teachey,Barbara L Kee,James LaBelle,Wendy Stock

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD32B (phospho Y292) antibody

Western blot - Anti-CD32B (phospho Y292) antibody (AB74092)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise