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AB74092

Anti-CD32B (phospho Y292) antibody

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(1 Review)

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(0 Publication)

Rabbit Polyclonal FCG2B phospho Y292 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FCGR2B phospho Y292.

View Alternative Names

CD32, FCG2, IGFR2, FCGR2B, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b

1 Images
Western blot - Anti-CD32B (phospho Y292) antibody (AB74092)
  • WB

Unknown

Western blot - Anti-CD32B (phospho Y292) antibody (AB74092)

All lanes:

Western blot - Anti-CD32B (phospho Y292) antibody (ab74092) at 1/500 dilution

Lane 1:

K562 cell extracts treated with PMA (125ng/ml, 30mins) at 30 µg

Lane 2:

K562 cell extracts treated with PMA (125ng/ml, 30mins) at 30 µg with immunising peptide

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human FCGR2B phospho Y292. The exact immunogen used to generate this antibody is proprietary information.

P31994

Specificity

ab74092 detects endogenous levels of CD32 only when phosphorylated at tyrosine 292.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab74092 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
See full target information FCGR2B phospho Y292
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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