AB68423

Anti-CD32B (phospho Y292) antibody [EP926Y]

RabMAb
Recombinant
What is this?

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(5 Publications)

Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) is a rabbit monoclonal antibody detecting CD32B in Western Blot, IP. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2008

View Alternative Names

CD32, FCG2, IGFR2, FCGR2B, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b

2 Images

Unknown

Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (AB68423)

Exposure time : 5 seconds

Observed MW : 40-44 KDa

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution

Lane 1:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 15 µg

Lane 2:

Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

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Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (AB68423)

ab68423 at 1/20 dilution immunoprecipitating CD32B in Raji (Human Burkitt's lymphoma) whole cell lysates.
Lane 1 (input) : Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
Lane 2 (+) : Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
For western blotting, ab68423 at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

Expsoure time : 1 second

Observed MW : 40-44 KDa

All lanes:

Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)

Predicted band size: 34 kDa

false

Carrier free

Anti-CD32B (phospho Y292) antibody [EP926Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP926Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/100000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human samples.

What is the molecular weight of CD32B?
Anti-CD32B (phospho Y292) [EP926Y] (ab68423) specifically detects a band for CD32B (UniProt: P31994) at a molecular weight of 40,44kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP926Y] also available for your convenience: ab68423, Carrier free - ab239861

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

See full target information FCGR2B phospho Y292

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biotechnology journal 19:e202400432 PubMed39655405

2024

A Competition-Based Strategy for the Isolation of an Anti-Idiotypic Blocking Module and Fine-Tuning for Conditional Activation of a Therapeutic Antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Habermann,Dominic Happel,Adrian Bloch,Charles Shin,Harald Kolmar

Cells 12: PubMed37681862

2023

Inhibitory Fc-Gamma IIb Receptor Signaling Induced by Multivalent IgG-Fc Is Dependent on Sialylation.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Beneduce,Stephanie Nguyen,Nathaniel Washburn,John Schaeck,Robin Meccariello,Kimberly Holte,Daniel Ortiz,Anthony M Manning,Carlos J Bosques,Elma Kurtagic

Immunology letters 160:145-50 PubMed24472604

2014

Characterization of SM201, an anti-hFcγRIIB antibody not interfering with ligand binding that mediates immune complex dependent inhibition of B cells.

Applications

Unspecified application

Species

Unspecified reactive species

N Rieth,A Carle,M A Müller,D ter Meer,C Direnberger,T Pohl,P Sondermann

Circulation research 109:1132-40 PubMed21940940

2011

Coupling of Fcγ receptor I to Fcγ receptor IIb by SRC kinase mediates C-reactive protein impairment of endothelial function.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan C Sundgren,Weifei Zhu,Ivan S Yuhanna,Ken L Chambliss,Mohamed Ahmed,Keiji Tanigaki,Michihisa Umetani,Chieko Mineo,Philip W Shaul

Journal of immunology (Baltimore, Md. : 1950) 186:4223-33 PubMed21357255

2011

Antibody-mediated coengagement of FcγRIIb and B cell receptor complex suppresses humoral immunity in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Holly M Horton,Seung Y Chu,Elizabeth C Ortiz,Erik Pong,Saso Cemerski,Irene W L Leung,Noam Jacob,Jonathan Zalevsky,John R Desjarlais,William Stohl,David E Szymkowski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD32B (phospho Y292) antibody [EP926Y]

Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (AB68423)

Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (AB68423)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

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