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AB203032

Anti-CD33 antibody

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(1 Publication)

Rabbit Polyclonal CD33 antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Cd33 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD33, Siglec3, Cd33, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3

2 Images
Flow Cytometry (Intracellular) - Anti-CD33 antibody (AB203032)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD33 antibody (AB203032)

Intracellular flow cytometric analysis of mouse splenocytes labeling CD33using ab203032 at 1/100 dilution followed by a PEconjugated secondary (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody (AB203032)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody (AB203032)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse intestine tissue labeling CD33 using ab203032 at a 1/200 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Cd33 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q63994

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "20-100 µg/mg of lysate", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (By similarity). Preferentially binds sialic acid to the short O-linked glycans of certain mucins (PubMed : 12773563).
See full target information Cd33
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7971801 PubMed36317105

2022

Acupuncture Inhibits Morphine Induced-Immune Suppress via Antioxidant System.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Jie Zhao,Dae Geon Lee,Chan Sik Park,Chae Ha Yang,Hee Young Kim,Mi Young Lee,Chang-Hyun Song,Il Je Cho,Sang Chan Kim,Sae Kwang Ku,Bong Hyo Lee
View all publications
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Product promise

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