AB203253

Anti-CD33 antibody

Name: Anti-CD33 antibody (ab203253) | Abcam
Brand: Abcam Limited
SKU: AB203253
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CD33 antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CD33 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody (AB203253)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody (AB203253)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue sections labeling CD33 with ab203253 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry (Intracellular) - Anti-CD33 antibody (AB203253)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD33 antibody (AB203253)

Intracellular Flow Cytometryanalysis of human U937 cells labeling CD33 with ab203253 (green).Control cells (blue), secondary only (light blue) and isotype control (orange).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human CD33 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P20138

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).

See full target information CD33

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40461158

2025

Tumor-intrinsic TTLL12 drives resistance to cancer immunotherapy via modulating myeloid-derived suppressor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Wen Chen,Yi-Kan Cheng,Pei-Si Li,Xiao-Jian Wu,Xin-Xin Huang,Jia-Hui Long,Zhizhong Xiong,Chong Chen,Shu-Biao Ye,Ping Lan

Frontiers in medicine 8:675363 PubMed34084773

2021

Enhanced mPGES-1 Contributes to PD-Related Peritoneal Fibrosis via Activation of the NLRP3 Inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Qimei Luo,Qinghua Hu,Qingkun Zheng,Lewei Gong,Lijuan Su,Baojun Ren,Yongle Ju,Zhanjun Jia,Xianrui Dou

Oncogene 40:1516-1530 PubMed33452462

2021

BATF2 prevents glioblastoma multiforme progression by inhibiting recruitment of myeloid-derived suppressor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhang,Yi Liu,Lei Dai,Gang Shi,Jie Deng,Qiang Luo,Qian Xie,Lin Cheng,Chunlei Li,Yi Lin,Qingnan Wang,Ping Fan,Hantao Zhang,Xiaolan Su,Shuang Zhang,Yang Yang,Xun Hu,Qiyong Gong,Dechao Yu,Lei Zheng,Hongxin Deng

Biomedicine & pharmacotherapy = Biomedecine & phar 112:108700 PubMed30970512

2019

Human telomerase reverse transcriptase recruits the β-catenin/TCF-4 complex to transactivate chemokine (C-C motif) ligand 2 expression in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Chen,Li Yang,Hui Dong,Hong Guo

View all publications

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