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AB30371

Anti-CD33 antibody [WM53]

5

(2 Reviews)

|

(3 Publications)

Mouse Recombinant Monoclonal CD33 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

2 Images
Flow Cytometry - Anti-CD33 antibody [WM53] (AB30371)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD33 antibody [WM53] (AB30371)

Flow cytometry staining of human whole blood with ab30371 (right) or mouse IgG1 and kappa; (ab170190) isotype (left). Red blood cells of 200 µL were lysed, then cells were incubated for 30 mins on ice in 1 x PBS containing 10 µg/mL human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interactions followed by antibody (ab30371) or mouse IgG1 & kappa; (ab170190) isotype for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor 488, Pre-absorbed) (ab150119) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD14.

Immunocytochemistry/ Immunofluorescence - Anti-CD33 antibody [WM53] (AB30371)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD33 antibody [WM53] (AB30371)

ab30371 staining CD33 in HL-60 cells. The cells were fixed with 4% paraformaldehyde (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab30371 at 10µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Carrier free

    Anti-CD33 antibody [WM53] - BSA and Azide free

  • 660 APC

    APC Anti-CD33 antibody [WM53]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD33 antibody [WM53]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD33 antibody [WM53]

  • 519 FITC

    FITC Anti-CD33 antibody [WM53]

  • 578 PE

    PE Anti-CD33 antibody [WM53]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD33 antibody [WM53]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD33 antibody [WM53]

  • 675 PerCP

    PerCP Anti-CD33 antibody [WM53]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WM53

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p>Works in both 4% PFA fixed cells (10mins) and 100% MeOH fixed cells (5mins)</p>" } } }
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Product details

This product has switched from a hybridoma to recombinant production method on 21st September 2020.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).
See full target information CD33
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC medicine 20:187 PubMed35550592

2022

Systematic analysis of IL-6 as a predictive biomarker and desensitizer of immunotherapy responses in patients with non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chengming Liu,Lu Yang,Haiyan Xu,Sufei Zheng,Zhanyu Wang,Sihui Wang,Yaning Yang,Shuyang Zhang,Xiaoli Feng,Nan Sun,Yan Wang,Jie He

Glia 69:1393-1412 PubMed33539598

2021

Deletion of Alzheimer's disease-associated CD33 results in an inflammatory human microglia phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Jannis Wißfeld,Ichiro Nozaki,Mona Mathews,Tamara Raschka,Christian Ebeling,Veit Hornung,Oliver Brüstle,Harald Neumann

Journal of immunological methods 361:1-20 PubMed20655312

2010

Highly multiparametric analysis by mass cytometry.

Applications

Flow Cyt

Species

Human

Olga Ornatsky,Dmitry Bandura,Vladimir Baranov,Mark Nitz,Mitchell A Winnik,Scott Tanner
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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