AB64480

Anti-CD34 antibody

(2 Reviews)

(10 Publications)

Rabbit Polyclonal CD34 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 10 publications.

View Alternative Names

CD34, Hematopoietic progenitor cell antigen CD34

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (AB64480)

IHC-P

AbReview20458****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (AB64480)

ab64480 staining CD34 in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/50) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (AB64480)

ab64480, at a 1/100 dilution, staining CD34 in formalin fixed, paraffin embedded human lung tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Alternative Products

Primary Antibodies

AB110643

Anti-CD34 antibody [EPR2999]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643)

Primary Antibodies

AB195013

Alexa Fluor® 488 Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (AB195013)

Primary Antibodies

AB195017

HRP Anti-CD34 antibody [EP373Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD34 antibody [EP373Y] (AB195017)

Primary Antibodies

AB223930

PE Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)

Primary Antibodies

AB81289

Anti-CD34 antibody [EP373Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (AB81289)

Primary Antibodies

AB198395

Anti-CD34 antibody [EP373Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (AB198395)

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

Primary Antibodies

AB28364

Anti-CD31 antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p>Use 10 mM sodium citrate pH 6.0, boiling for 10 minutes followed by 20 minutes cooling.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

style

left-column

Target data

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

See full target information CD34

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2021:5599364 PubMed34456628

2021

p300/Sp1-Mediated High Expression of p16 Promotes Endothelial Progenitor Cell Senescence Leading to the Occurrence of Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui He,Huaihuai Peng,Min Gao,Guibin Liang,Menghao Zeng,Xuefeng Zhang

Frontiers in oncology 10:705 PubMed32714856

2020

Pro-Angiogenic and Pro-Inflammatory Regulation by lncRNA MCM3AP-AS1-Mediated Upregulation of DPP4 in Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Qiu,Yan Ma,Yanming Yang,Xiaojun Ren,Dongzhou Wang,Xiaojing Jia

Acta biomaterialia 94:495-504 PubMed31252171

2019

cRGD-functionalized nanoparticles for combination therapy of anti-endothelium dependent vessels and anti-vasculogenic mimicry to inhibit the proliferation of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yifeng Wang,Lin Tong,Jianguo Wang,Jiamao Luo,Jiao Tang,Lijuan Zhong,Qian Xiao,Wenbo Niu,Jinheng Li,Junqiao Zhu,Huajian Chen,Xin Li,Ying Wang

Journal of cellular physiology 234:692-708 PubMed30132846

2018

Molecular characterization of autophagic and apoptotic signaling induced by sorafenib in liver cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

María A Rodríguez-Hernández,Raúl González,Ángel J de la Rosa,Paloma Gallego,Raquel Ordóñez,Elena Navarro-Villarán,Laura Contreras,Mario Rodríguez-Arribas,Javier González-Gallego,José M Álamo-Martínez,Luís M Marín-Gómez,José A Del Campo,José L Quiles,José M Fuentes,Jesús de la Cruz,José L Mauriz,Francisco J Padillo,Jordi Muntané

Journal of pathology and translational medicine 50:361-8 PubMed27498544

2016

CD99 Is Strongly Expressed in Basal Cells of the Normal Adult Epidermis and Some Subpopulations of Appendages: Comparison with Developing Fetal Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Gawon Choi,Jin Roh,Chan-Sik Park

The American journal of sports medicine 43:2411-22 PubMed26311443

2015

FGF-2 Stimulates the Growth of Tenogenic Progenitor Cells to Facilitate the Generation of Tenomodulin-Positive Tenocytes in a Rat Rotator Cuff Healing Model.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Tokunaga,Chisa Shukunami,Nobukazu Okamoto,Takuya Taniwaki,Kiyoshi Oka,Hidetoshi Sakamoto,Junji Ide,Hiroshi Mizuta,Yuji Hiraki

PloS one 9:e97488 PubMed24828425

2014

Effects of acupuncture at GV20 and ST36 on the expression of matrix metalloproteinase 2, aquaporin 4, and aquaporin 9 in rats subjected to cerebral ischemia/reperfusion injury.

Applications

ICC/IF

Species

Rat

Hong Xu,Yamin Zhang,Hua Sun,Suhui Chen,Fuming Wang

World journal of surgery 38:2089-96 PubMed24663482

2014

Intrahepatic lymphatic invasion but not vascular invasion is a major prognostic factor after resection of colorectal cancer liver metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Renato Micelli Lupinacci,Evandro Sobrosa Mello,Rafael S Pinheiro,Gilton Marques,Fabrício Ferreira Coelho,Jaime Arthur Pirolla Kruger,Marcos Vinícius Perini,Paulo Herman

Placenta 31:1120-2 PubMed20947164

2010

Pericyte coverage of fetoplacental vessels in pregnancies complicated by Type 1 diabetes mellitus.

Applications

IHC-P

Species

Human

T Kučera,I Vyletěl,M Moravcová,V Krejčí,Z Zižka,M Jirkovská

The American journal of pathology 175:2668-75 PubMed19893052

2009

Septoclast deficiency accompanies postnatal growth plate chondrodysplasia in the toothless (tl) osteopetrotic, colony-stimulating factor-1 (CSF-1)-deficient rat and is partially responsive to CSF-1 injections.

Applications

IHC-P

Species

Mouse

Alison Gartland,April Mason-Savas,Meiheng Yang,Carole A MacKay,Mark J Birnbaum,Paul R Odgren

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com