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Anti-CD34 antibody [EP373Y] (ab81289) is a rabbit monoclonal antibody detecting CD34 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 570 publications

- Trusted since 2009


Images

Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (AB81289), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (AB81289), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (AB81289), expandable thumbnail
  • Immunoprecipitation - Anti-CD34 antibody [EP373Y] (AB81289), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (AB81289), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFIHC-FrFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Expected
Tested
Mouse
Tested
Expected
Tested
Expected
Not recommended
Expected
Rat
Tested
Expected
Expected
Expected
Tested
Expected
African bush elephant
Predicted
Predicted
Predicted
Predicted
Not recommended
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Not recommended
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Not recommended
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/2500 - 1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2500 - 1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2500 - 1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Sheep, Dog, African bush elephant
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Dog, African bush elephant
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

ab81289 is not suitable for rat species in WB application.

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Dog, African bush elephant
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Dog, African bush elephant
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/50
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Sheep, Dog, African bush elephant
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/11550
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Dog, African bush elephant
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Alternative names

Recommended products

Anti-CD34 antibody [EP373Y] (ab81289) is a rabbit monoclonal antibody detecting CD34 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 570 publications

- Trusted since 2009

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP373Y
Purification technique
Affinity purification Protein A
Dissociation constant
1.15 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-CD34 antibody [EP373Y] (ab81289) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P and IP.

Anti-CD34 antibody [EP373Y] (ab81289) was first used in a scientific publication in 2010 and has been cited over 570 times in peer reviewed journals. It's performance in IHC and Flow Cytometry in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-CD34 antibody [EP373Y] (ab81289) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-CD34 antibody [EP373Y] (ab81289) has 54 independent reviews from customers.

Anti-CD34 antibody [EP373Y] (ab81289) specifically detects CD34 (UniProt ID: P28906; Molecular weight: 38kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EP373Y - ab188169.

Antibody clone EP373Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, HRP, PE, APC, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750 (Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] ab195013, HRP Anti-CD34 antibody [EP373Y] ab195017, PE Anti-CD34 antibody [EP373Y] ab223930, APC Anti-CD34 antibody [EP373Y] ab310881, Alexa Fluor® 594 Anti-CD34 antibody [EP373Y] ab311670, Alexa Fluor® 568 Anti-CD34 antibody [EP373Y] ab312945, Alexa Fluor® 555 Anti-CD34 antibody [EP373Y] ab313155, Alexa Fluor® 750 Anti-CD34 antibody [EP373Y] ab321739).

CD34 is a marker for hematopoietic stem cells and plays a role in immunology diseases by facilitating cell migration and adhesion, impacting conditions like leukaemia and other hematologic disorders. EP373Y is top cited on the market with over 763 citations and validated in human, mouse, rat samples with the broadest range of applications.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (ab81289)

    Intracellular Flow Cytometry analysis of TF-1 (human erythroleukemia) cells labeling CD34 with purified ab81289 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail
    This image is courtesy of a customer review submitted by Rudolf Jung.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue stained for CD34 using ab81289 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Paraformaldehyde-fixed, paraffin-embedded mouse prostate tissue stained for CD34 using ab81289 at 1/150 dilution in immunohistochemical analysis.

  • Immunoprecipitation - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Immunoprecipitation - Anti-CD34 antibody [EP373Y] (ab81289)

    CD34 was immunoprecipitated from TF-1 (Human bone marrow erythroleukemia cells) cells using purified ab81289 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab81289. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-CD34 antibody [EP373Y] (ab81289)

    Predicted band size: 40 kDa

    Observed band size: 120 kDa

  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling CD34 with ab81289 at 1/50 (11.04 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat kidney. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling CD34 with ab81289 at 1/50 (11.04 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat lung. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.

    Control: Secondary antibody only, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

  • OI-RD Scanning - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    OI-RD Scanning - Anti-CD34 antibody [EP373Y] (ab81289)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] (ab81289)

    IHC image of CD34 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab81289, 1/250 dilution, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail
    Image from Földes G et al. PLoS One. 2010 May 5;5(5):e10501 Fig 3.

    Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] (ab81289)

    CD34 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-CD34 antibody

    Immunocytochemistry/Immunofluorescence analysis of human embryonic stem cell-derived endothelial cells labeling CD34 with unpurified ab81289 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% aTriton X-100. An Alexa Fluor® 647-conjugated secondary antibody was used at a 1/400 dilution. DAPI (blue) was used as the nuclear counter stain.

  • Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (ab81289)

    Flow cytometry overlay histogram showing left HUVEC positive cells and right negative HL60 stained with ab81289 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 mins. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab81289) (1x 106 in 100μl at 0.2μg/ml (1/11550 dilution)) for 30 mins at 22°C.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 dilution for 30 mins at 22°C

    Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Western blot - Anti-CD34 antibody [EP373Y] (ab81289), expandable thumbnail

    Western blot - Anti-CD34 antibody [EP373Y] (ab81289)

    CD34 Western blot staining using rabbit Anti-CD34 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Daudi, HL-60.

    Low expression: HUVEC, Neuro-2a.

    All lanes: Western blot - Anti-CD34 antibody [EP373Y] (ab81289) at 1/1000 dilution

    Lane 1: KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg

    Lane 2: TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg

    Lane 3: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 4: HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 5: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 6: bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg

    Lane 7: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 8: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 80-120 kDa

    Exposure time: 60s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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