Anti-CD34 antibody [EPR27431-71]

16 product images

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  Multiplex immunohistochemistry - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections mouse stomach tissue labelling Sodium/Hydrogen Exchanger 1/NHE-1 with Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] ab322349 at 1/4000 dilution (B), CD34 with ab316277 at 1/10000 dilution (C) and Cathepsin E with ab322657 at 1/5000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 1/NHE-1 (magenta; Opal™690), anti-CD34 (green; Opal™520) and anti-Cathepsin E (gray; Opal™570) on mouse stomach.

  Panel B: anti-Sodium/Hydrogen Exchanger 1/NHE-1 showed membranous staining in epithelium of mouse stomach.

  Panel C: anti-CD34 staining endothelium in mouse stomach.

  Panel D: anti-Cathepsin E showed cytoplasmic staining in mouse stomach.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody [EPR28913-51] ab322349, ab316277 and ab322657 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Flow Cytometry - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Flow cytometric analysis of Mouse bone marrow cells labelling CD34 with ab316277 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  Gated on viable cells. Cells are co-stained with CD117 conjugated to APC-eFluor 780. (PMID: 23295606)

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  Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CD34 with ab316277 at 1/100 (5.09 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing membranous staining in NIH/3T3 cell line. Negative control: Neuro-2a. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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  Western blot - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Low expression tissue: liver (PMID:1709048).

  The identity of the bands between 25 kDa and 37 kDa in lane 4 are unknown.

  The lanes 2-4 were developed using a high sensitivity ECL substrate.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  Exposure time: Lane 1: 59 seconds, lanes 2-4: 180 seconds

  All lanes: Western blot - Anti-CD34 antibody [EPR27431-71] (ab316277) at 1/1000 dilution

  Lane 1: Mouse lung tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 2: Mouse placenta tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 3: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 4: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 80 kDa, 36 kDa

• 

  Western blot - Anti-CD34 antibody [EPR27431-71] (ab316277)

  This antibody doesn't detect with de-glycosylated form of CD34.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-CD34 antibody [EPR27431-71] (ab316277) at 1/1000 dilution

  Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 2: NIH/3T3 treated with Peptide:N-glycosidase F (PNGase F) whole cell lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 80 kDa, 36 kDa

  Exposure time: 180s

• 

  Western blot - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Negative control: Neuro-2a.

  In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

  All lanes: Western blot - Anti-CD34 antibody [EPR27431-71] (ab316277) at 1/1000 dilution

  Lane 1: bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 80 kDa, 15 kDa

  Exposure time: 59s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: weak staining on endothelial cells of rat liver. The section was incubated with ab316277 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: weak staining on endothelial cells of mouse liver (PMID:1709048). The section was incubated with ab316277 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on basement membrane of rat testis. The section was incubated with ab316277 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of rat kidney. The section was incubated with ab316277 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of rat cerebrum. The section was incubated with ab316277 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Mouse squamouse cell carcinoma tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of mouse squamouse cell carcinoma. The section was incubated with ab316277 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on basement membrane of mouse testis (PMID:1709048). The section was incubated with ab316277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of mouse kidney. The section was incubated with ab316277 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD34 with ab316277 at 1/8000 (0.064 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of mouse spleen (PMID:1709048). The section was incubated with ab316277 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Flow Cytometry - Anti-CD34 antibody [EPR27431-71] (ab316277)

  Flow cytometric analysis of Neuro-2a (mouse neuroblastoma neuroblast, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling CD34 with ab316277 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  Negative control: Neuro-2a.