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Human Recombinant Monoclonal CD34 antibody. Suitable for ICC/IF, IHC-P and reacts with Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) (AB323246), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) (AB323246), expandable thumbnail

Key facts

Isotype
IgG
Host species
Human
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-P
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins (By similarity).

Alternative names

CD34, Hematopoietic progenitor cell antigen CD34, 2

Human Recombinant Monoclonal CD34 antibody. Suitable for ICC/IF, IHC-P and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27431-71
Purification technique
Affinity purification Protein A
Specificity

IHC-P showed non-specific staining on mouse cardiac muscle and mouse brain.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This human monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-CD34 antibody [EPR27431-71] ab316277). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) (ab323246), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) (ab323246)

    Immunofluorescence staining of CD34 in sections of formalin-fixed paraffin-embedded mouse lung (positive) and mouse liver (negative).Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab323246 at 1/100 dilution and then incubated for 1 hour with Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) ab200622 Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) (ab323246), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) (ab323246)

    ab323246 staining CD34 in NIH/3T3 (positive) and Neuro-2a (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal rabbit serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with ab323246 at 5ug/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) ab200622 Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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