Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) – BSA and Azide Free
- BOND RX™ Validated
- Recombinant
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Human Recombinant Monoclonal CD34 antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Mouse samples.
View Alternative Names
CD34, Hematopoietic progenitor cell antigen CD34, 2
![Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) – BSA and Azide Free (AB323255)](https://content.abcam.com/products/images/cd34-antibody-epr27431-71-human-igg-chimeric-bsa-and-azide-free-ab323255--immunocytochemistry-immunofluorescence-img440859.jpg/_jcr_content/renditions/webp-768.webp)
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) – BSA and Azide Free (AB323255)
ab323246 staining CD34 in NIH/3T3 (positive) and TRA-differentiated Neuro2a (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal rabbit serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with ab323246 at 5ug/ml (shown in green) and ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab200622 Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This data was developed using the same antibody clone in a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) – BSA and Azide Free (AB323255)](https://content.abcam.com/products/images/cd34-antibody-epr27431-71-human-igg-chimeric-bsa-and-azide-free-ab323255--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440860.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR27431-71] – Human IgG (Chimeric) – BSA and Azide Free (AB323255)
Immunofluorescence staining of CD34 in sections of formalin-fixed paraffin-embedded mouse lung (positive) and mouse liver (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab323246 at 1/100 dilution and then incubated for 1 hour with ab200622 Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
This data was developed using the same antibody clone in a different buffer formulation.
Reactivity data
Product details
This human monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab316277). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
ab323255 is the carrier-free version of ab323246
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Product protocols
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Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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